Source:http://linkedlifedata.com/resource/pubmed/id/18854171
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions |
umls-concept:C0004112,
umls-concept:C0010453,
umls-concept:C0018042,
umls-concept:C0031715,
umls-concept:C0205225,
umls-concept:C0292777,
umls-concept:C1514562,
umls-concept:C1514873,
umls-concept:C1546857,
umls-concept:C1556066,
umls-concept:C1619636,
umls-concept:C1705165,
umls-concept:C1707271,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221,
umls-concept:C2700061
|
pubmed:issue |
2
|
pubmed:dateCreated |
2008-11-14
|
pubmed:abstractText |
Aquaporin-4 (AQP4) is expressed in the perivascular and subpial astrocytes end-feet in mammalian brain, and plays a critical component of an integrated water and potassium homeostasis. Here we examine whether AQP4 is phosphorylated in primary cultured mouse astrocytes. Astrocytes were metabolically labeled with [(32)P]phosphoric acid, then AQP4 was immunoprecipitated with anti-AQP4 antibody. We observed that AQP4 was constitutively phosphorylated, which is reduced by treatment with protein kinase CK2 inhibitors. To elucidate the phosphorylation of AQP4 by CK2, myc-tagged wild-type or mutant AQP4 was transiently transfected in primary cultured astrocytes. Substitution of Ala residues for four putative CK2 phosphorylation sites in the C terminus abolished the phosphorylation of AQP4. Immunofluorescent microscopy revealed that the quadruple mutant was localized in the Golgi apparatus. These observations indicate that the C-terminal domain of AQP4 is constitutively phosphorylated at least in part by protein kinase CK2 and it is required for Golgi transition.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
|
pubmed:issn |
1090-2104
|
pubmed:author | |
pubmed:issnType |
Electronic
|
pubmed:day |
12
|
pubmed:volume |
377
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
463-8
|
pubmed:dateRevised |
2009-11-19
|
pubmed:meshHeading |
pubmed-meshheading:18854171-Amino Acid Sequence,
pubmed-meshheading:18854171-Amino Acid Substitution,
pubmed-meshheading:18854171-Animals,
pubmed-meshheading:18854171-Aquaporin 4,
pubmed-meshheading:18854171-Astrocytes,
pubmed-meshheading:18854171-Casein Kinase II,
pubmed-meshheading:18854171-Golgi Apparatus,
pubmed-meshheading:18854171-Humans,
pubmed-meshheading:18854171-Mice,
pubmed-meshheading:18854171-Phosphorylation,
pubmed-meshheading:18854171-Protein Structure, Tertiary,
pubmed-meshheading:18854171-Protein Transport
|
pubmed:year |
2008
|
pubmed:articleTitle |
Phosphorylation in the C-terminal domain of Aquaporin-4 is required for Golgi transition in primary cultured astrocytes.
|
pubmed:affiliation |
Department of Pharmacology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|