Source:http://linkedlifedata.com/resource/pubmed/id/18853211
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
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pubmed:dateCreated |
2008-10-30
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pubmed:abstractText |
Gene expression analyses with real-time (RT)-polymerase chain reaction (PCR) gains importance in marine monitoring. This new technique has to be compared to the classical approaches like the well known biomarker ethoxyresorufin-O-deethylase (EROD) to test their suitability for monitoring programmes. The goal of the present study is to compare EROD activity and CYP1A1 mRNA expression in the important monitoring fish species dab (Limanda limanda) and to answer the question of whether these parameters reflect the polycyclic aromatic hydrocarbon (PAH) contamination of the fish. Further on, glyceraldehyd-3-phosphate dehydrogenase (GAPDH) was investigated as a potential housekeeping gene.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0944-1344
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
600-5
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pubmed:dateRevised |
2009-8-14
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pubmed:meshHeading |
pubmed-meshheading:18853211-Animals,
pubmed-meshheading:18853211-Body Size,
pubmed-meshheading:18853211-Cytochrome P-450 CYP1A1,
pubmed-meshheading:18853211-Environmental Monitoring,
pubmed-meshheading:18853211-Female,
pubmed-meshheading:18853211-Fishes,
pubmed-meshheading:18853211-Germany,
pubmed-meshheading:18853211-Humans,
pubmed-meshheading:18853211-Male,
pubmed-meshheading:18853211-Oceans and Seas,
pubmed-meshheading:18853211-RNA, Messenger,
pubmed-meshheading:18853211-Seasons,
pubmed-meshheading:18853211-Transcription, Genetic
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pubmed:year |
2008
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pubmed:articleTitle |
Biological effect monitoring in dab (Limanda limanda) using gene transcript of CYP1A1 or EROD-a comparison.
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pubmed:affiliation |
Institute for Fishery Ecology, Johann Heinrich von Thünen-Institut (vTI), Federal Research Institute for Rural Areas, Forests and Fisheries, Palmaille 9, 22767 Hamburg, Germany. ulrike.kammann@vti.bund.de
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pubmed:publicationType |
Journal Article
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