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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2008-10-28
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pubmed:abstractText |
RNA isolation and purification steps greatly influence the results of gene expression profiling. There are two commercially available products for whole blood RNA collection, PAXgene and Tempus blood collection tubes, and each comes with their own RNA purification method. In both systems the blood is immediately lysed when collected into the tube and RNA stabilized using proprietary reagents. Both systems enable minimal blood handling procedures thus minimizing the risk of inducing changes in gene expression through blood handling or processing. Because the RNA purification steps could influence the total RNA pool, we examined the impact of RNA isolation, using the PAXgene or Tempus method, on gene expression profiles.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18847473-11226464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18847473-11682119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18847473-12073021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18847473-12184808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18847473-12406972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18847473-12454744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18847473-12613259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18847473-12624187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18847473-14636415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18847473-14647279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18847473-14659918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18847473-15113097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18847473-15461798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18847473-15548831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18847473-15723591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18847473-15797563
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1471-2164
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
474
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2008
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pubmed:articleTitle |
Differential gene expression profiles are dependent upon method of peripheral blood collection and RNA isolation.
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pubmed:affiliation |
University of California, San Francisco, Immune Tolerance Network, 3 Bethesda Metro Suite 400, Bethesda, MD 20814, USA. aasare@immunetolerance.org
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
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