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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2008-11-4
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pubmed:abstractText |
The pancreatic islets of Langerhans are highly vascularized micro-organs that play a key role in the regulation of blood glucose homeostasis. The specific arrangement of endocrine cell types in islets suggests a coupling between morphology and function within the islet. Here, we established a line-scanning confocal microscopy approach to examine the relationship between blood flow and islet cell type arrangement by real-time in vivo imaging of intra-islet blood flow in mice. These data were used to reconstruct the in vivo 3D architecture of the islet and time-resolved blood flow patterns throughout the islet vascular bed. The results revealed 2 predominant blood flow patterns in mouse islets: inner-to-outer, in which blood perfuses the core of beta cells before the islet perimeter of non-beta cells, and top-to-bottom, in which blood perfuses the islet from one side to the other regardless of cell type. Our approach included both millisecond temporal resolution and submicron spatial resolution, allowing for real-time imaging of islet blood flow within the living mouse, which has not to our knowledge been attainable by other methods.
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pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/CA68485,
http://linkedlifedata.com/resource/pubmed/grant/DK07563,
http://linkedlifedata.com/resource/pubmed/grant/DK20593,
http://linkedlifedata.com/resource/pubmed/grant/DK53434,
http://linkedlifedata.com/resource/pubmed/grant/DK58404,
http://linkedlifedata.com/resource/pubmed/grant/DK59637,
http://linkedlifedata.com/resource/pubmed/grant/DK63439,
http://linkedlifedata.com/resource/pubmed/grant/DK66636,
http://linkedlifedata.com/resource/pubmed/grant/DK68764,
http://linkedlifedata.com/resource/pubmed/grant/DK69603,
http://linkedlifedata.com/resource/pubmed/grant/EY08126,
http://linkedlifedata.com/resource/pubmed/grant/HD15052,
http://linkedlifedata.com/resource/pubmed/grant/RR22620
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-11052970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-12107718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-12388130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-12415310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-1482604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-15919801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-15923354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-16399504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-16871621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-17065332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-17065333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-17179392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-17347899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-17803903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-18327249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-18418560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-1969425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-1976222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-2455737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-2568960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-2903966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-3512599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-6385219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-6759221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-8238968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-8373657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-8419903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-8603757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18846254-9861040
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
118
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3790-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:18846254-Animals,
pubmed-meshheading:18846254-Diagnostic Imaging,
pubmed-meshheading:18846254-Hemodynamics,
pubmed-meshheading:18846254-Imaging, Three-Dimensional,
pubmed-meshheading:18846254-Islets of Langerhans,
pubmed-meshheading:18846254-Mice,
pubmed-meshheading:18846254-Mice, Transgenic,
pubmed-meshheading:18846254-Microscopy, Confocal,
pubmed-meshheading:18846254-Time Factors
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pubmed:year |
2008
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pubmed:articleTitle |
Real-time, multidimensional in vivo imaging used to investigate blood flow in mouse pancreatic islets.
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pubmed:affiliation |
Department of Medicine, Vanderbilt University, Nashville, Tennessee, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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