Source:http://linkedlifedata.com/resource/pubmed/id/18844289
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
12
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pubmed:dateCreated |
2008-12-16
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pubmed:abstractText |
This study describes the activity-guided isolation and purification of a novel antimicrobial protein from the seed of Croton tiglium Linn. Purification was carried out by (NH(4))(2)SO(4) precipitation, gel filtration and DEAE-cellulose ion-exchange chromatography. Antifungal and antibacterial activities were determined after each purification step. SDS-polyacrylamide gel electrophoresis revealed that the purified protein was a monomer with molecular mass of 50 kDa. This is a first report on purification of a protein from Croton tiglium, which possesses a strong and broad spectrum antimicrobial activity.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1099-1573
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pubmed:author | |
pubmed:copyrightInfo |
(c) 2008 John Wiley & Sons, Ltd.
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pubmed:issnType |
Electronic
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1646-9
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pubmed:meshHeading |
pubmed-meshheading:18844289-Anti-Bacterial Agents,
pubmed-meshheading:18844289-Antifungal Agents,
pubmed-meshheading:18844289-Chromatography, Ion Exchange,
pubmed-meshheading:18844289-Croton,
pubmed-meshheading:18844289-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:18844289-Microbial Sensitivity Tests,
pubmed-meshheading:18844289-Molecular Weight,
pubmed-meshheading:18844289-Plant Extracts,
pubmed-meshheading:18844289-Seeds
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pubmed:year |
2008
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pubmed:articleTitle |
Activity-guided isolation of a novel protein from Croton tiglium with antifungal and antibacterial activities.
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pubmed:affiliation |
Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad-38040, Pakistan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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