Linked Life Data

18842903

Source:http://linkedlifedata.com/resource/pubmed/id/18842903

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
41
pubmed:dateCreated
2008-10-9
pubmed:abstractText
Despite their abundance, still little is known about the rather frequent, constantly proliferating progenitors spread throughout the adult mouse brain parenchyma. The majority of these progenitors express the basic-helix-loop-helix transcription factor Olig2, and their number further increases after injury. Here, we examine the progeny of this progenitor population by genetic fate mapping using tamoxifen-inducible Cre-recombination in the Olig2 locus to turn on permanent reporter gene expression in the adult brain. Consistent with Olig2 expression in proliferating NG2(+) progenitors, most reporter(+) cells seen shortly after initiating recombination at adult stages incorporated BrdU and contained the proteoglycan NG2 in both the gray (GM) and the white matter (WM) of the cerebral cortex. However, at longer time points after induction, we observed profound differences in the identity of reporter(+) cells in the WM and GM. Whereas most of the Olig2(+) progenitors had generated mature, myelinating oligodendrocytes in the WM, hardly any reporter(+) cells showing mature oligodendrocyte characteristics were detectable even up to 6 months after recombination in the GM. In the GM, most reporter(+) cells remained NG2(+), even after injury, but stopped proliferating rather soon after recombination. Thus, our results demonstrate the continuous generation of mature, myelinating oligodendrocytes in the WM, whereas cells in the GM generated mostly postmitotic NG2(+) glia.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
8
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10434-42
pubmed:meshHeading
pubmed-meshheading:18842903-Animals, pubmed-meshheading:18842903-Animals, Genetically Modified, pubmed-meshheading:18842903-Antigens, pubmed-meshheading:18842903-Basic Helix-Loop-Helix Transcription Factors, pubmed-meshheading:18842903-Cell Differentiation, pubmed-meshheading:18842903-Cell Proliferation, pubmed-meshheading:18842903-Cerebral Cortex, pubmed-meshheading:18842903-Gene Expression, pubmed-meshheading:18842903-Genes, Reporter, pubmed-meshheading:18842903-Integrases, pubmed-meshheading:18842903-Mice, pubmed-meshheading:18842903-Mice, Inbred C57BL, pubmed-meshheading:18842903-Mitosis, pubmed-meshheading:18842903-Myelin Sheath, pubmed-meshheading:18842903-Nerve Tissue Proteins, pubmed-meshheading:18842903-Neuroglia, pubmed-meshheading:18842903-Oligodendroglia, pubmed-meshheading:18842903-Periaqueductal Gray, pubmed-meshheading:18842903-Proteoglycans, pubmed-meshheading:18842903-Stem Cells, pubmed-meshheading:18842903-Tamoxifen, pubmed-meshheading:18842903-Wounds, Stab
pubmed:year
2008
pubmed:articleTitle
Progeny of Olig2-expressing progenitors in the gray and white matter of the adult mouse cerebral cortex.
pubmed:affiliation
Department of Physiological Genomics, Institute of Physiology, Ludwig-Maximilians University Munich, 80336 Munich, Germany. leda.dimou@lrz.uni-muenchen.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't