| pubmed-article:18842822 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18842822 | lifeskim:mentions | umls-concept:C0542341 | lld:lifeskim |
| pubmed-article:18842822 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:18842822 | lifeskim:mentions | umls-concept:C1414243 | lld:lifeskim |
| pubmed-article:18842822 | lifeskim:mentions | umls-concept:C0439799 | lld:lifeskim |
| pubmed-article:18842822 | lifeskim:mentions | umls-concept:C0456962 | lld:lifeskim |
| pubmed-article:18842822 | lifeskim:mentions | umls-concept:C1701901 | lld:lifeskim |
| pubmed-article:18842822 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:18842822 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:18842822 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:18842822 | pubmed:dateCreated | 2008-12-31 | lld:pubmed |
| pubmed-article:18842822 | pubmed:abstractText | A recently described novel controllable method to regulate protein expression is based on a mutated FK506-binding protein-12 (mtFKBP) that is unstable and rapidly degraded in mammalian cells. This instability can be conferred to other proteins directly fused to mtFKBP. Binding of a synthetic cell-permeant ligand (Shield-1) to mtFKBP reverses the instability, allowing conditional expression of mtFKBP-fused proteins. We adapted this strategy to study multimeric plasma membrane proteins using the ion channel TRPV5 as model protein. mtFKBP-TRPV5 forms functional ion channels and its expression can be controlled in a time- and dose-dependent fashion using Shield-1. Moreover, in the presence of Shield-1, mtFKBP-TRPV5 formed heteromultimeric channels with untagged TRPV5, which were codegraded upon washout of Shield-1, providing a strategy to study multimeric plasma membrane protein complexes without the need to destabilize all individual subunits. | lld:pubmed |
| pubmed-article:18842822 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18842822 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18842822 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18842822 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18842822 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18842822 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18842822 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18842822 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18842822 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18842822 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18842822 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18842822 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:18842822 | pubmed:issn | 1931-857X | lld:pubmed |
| pubmed-article:18842822 | pubmed:author | pubmed-author:BindelsRené... | lld:pubmed |
| pubmed-article:18842822 | pubmed:author | pubmed-author:HoenderopJoos... | lld:pubmed |
| pubmed-article:18842822 | pubmed:author | pubmed-author:LeeKyu PilKP | lld:pubmed |
| pubmed-article:18842822 | pubmed:author | pubmed-author:van de... | lld:pubmed |
| pubmed-article:18842822 | pubmed:author | pubmed-author:WittgenHannek... | lld:pubmed |
| pubmed-article:18842822 | pubmed:author | pubmed-author:SchoeberJoost... | lld:pubmed |
| pubmed-article:18842822 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:18842822 | pubmed:volume | 296 | lld:pubmed |
| pubmed-article:18842822 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18842822 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18842822 | pubmed:pagination | F204-11 | lld:pubmed |
| pubmed-article:18842822 | pubmed:dateRevised | 2011-4-28 | lld:pubmed |
| pubmed-article:18842822 | pubmed:meshHeading | pubmed-meshheading:18842822... | lld:pubmed |
| pubmed-article:18842822 | pubmed:meshHeading | pubmed-meshheading:18842822... | lld:pubmed |
| pubmed-article:18842822 | pubmed:meshHeading | pubmed-meshheading:18842822... | lld:pubmed |
| pubmed-article:18842822 | pubmed:meshHeading | pubmed-meshheading:18842822... | lld:pubmed |
| pubmed-article:18842822 | pubmed:meshHeading | pubmed-meshheading:18842822... | lld:pubmed |
| pubmed-article:18842822 | pubmed:meshHeading | pubmed-meshheading:18842822... | lld:pubmed |
| pubmed-article:18842822 | pubmed:meshHeading | pubmed-meshheading:18842822... | lld:pubmed |
| pubmed-article:18842822 | pubmed:meshHeading | pubmed-meshheading:18842822... | lld:pubmed |
| pubmed-article:18842822 | pubmed:meshHeading | pubmed-meshheading:18842822... | lld:pubmed |
| pubmed-article:18842822 | pubmed:meshHeading | pubmed-meshheading:18842822... | lld:pubmed |
| pubmed-article:18842822 | pubmed:meshHeading | pubmed-meshheading:18842822... | lld:pubmed |
| pubmed-article:18842822 | pubmed:meshHeading | pubmed-meshheading:18842822... | lld:pubmed |
| pubmed-article:18842822 | pubmed:meshHeading | pubmed-meshheading:18842822... | lld:pubmed |
| pubmed-article:18842822 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:18842822 | pubmed:articleTitle | Conditional fast expression and function of multimeric TRPV5 channels using Shield-1. | lld:pubmed |
| pubmed-article:18842822 | pubmed:affiliation | Dept. of Physiology (286), Nijmegen Centre for Molecular Life Sciences, Radboud Univ. Nijmegen Medical Centre, Nijmegen 6500 HB, The Netherlands. | lld:pubmed |
| pubmed-article:18842822 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18842822 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18842822 | lld:pubmed |
