Linked Life Data

18842822

Source:http://linkedlifedata.com/resource/pubmed/id/18842822

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-12-31
pubmed:abstractText
A recently described novel controllable method to regulate protein expression is based on a mutated FK506-binding protein-12 (mtFKBP) that is unstable and rapidly degraded in mammalian cells. This instability can be conferred to other proteins directly fused to mtFKBP. Binding of a synthetic cell-permeant ligand (Shield-1) to mtFKBP reverses the instability, allowing conditional expression of mtFKBP-fused proteins. We adapted this strategy to study multimeric plasma membrane proteins using the ion channel TRPV5 as model protein. mtFKBP-TRPV5 forms functional ion channels and its expression can be controlled in a time- and dose-dependent fashion using Shield-1. Moreover, in the presence of Shield-1, mtFKBP-TRPV5 formed heteromultimeric channels with untagged TRPV5, which were codegraded upon washout of Shield-1, providing a strategy to study multimeric plasma membrane protein complexes without the need to destabilize all individual subunits.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1931-857X
pubmed:author
pubmed:issnType
Print
pubmed:volume
296
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
F204-11
pubmed:dateRevised
2011-4-28
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Conditional fast expression and function of multimeric TRPV5 channels using Shield-1.
pubmed:affiliation
Dept. of Physiology (286), Nijmegen Centre for Molecular Life Sciences, Radboud Univ. Nijmegen Medical Centre, Nijmegen 6500 HB, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't