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rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0021665,
umls-concept:C0063674,
umls-concept:C0085080,
umls-concept:C0086418,
umls-concept:C0185117,
umls-concept:C0205419,
umls-concept:C1327616,
umls-concept:C1880022,
umls-concept:C1998793,
umls-concept:C2911684
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|
pubmed:issue |
3
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|
pubmed:dateCreated |
1991-10-7
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|
pubmed:abstractText |
Recombinant human insulin-like growth factor-I (hIGF-I) and a biologically potent variant lacking the N-terminal tripeptide (des(1-3)IGF-I) were produced from transfected Chinese hamster ovary cells. The constructs encoding the signal peptide, sequence of the mature peptide and a C-terminal extension peptide were expressed under the control of a Rous sarcoma virus promoter. Successfully transfected clones secreting correctly processed recombinant hIGF-I or des(1-3)IGF-I were selected by their secretion of IGF-I-like activity into the culture medium. The recombinant peptides were purified to homogeneity as assessed by high-performance liquid chromatography and N-terminal sequence analysis. The purified recombinant peptides exhibited biological potencies equivalent to authentic IGF-I and des(1-3)IGF-I respectively.
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pubmed:language |
eng
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pubmed:journal |
|
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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|
pubmed:month |
Jun
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pubmed:issn |
0952-5041
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|
pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
6
|
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
231-9
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|
pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:1883485-Amino Acid Sequence,
pubmed-meshheading:1883485-Animals,
pubmed-meshheading:1883485-Base Sequence,
pubmed-meshheading:1883485-Cell Line,
pubmed-meshheading:1883485-Chromatography, High Pressure Liquid,
pubmed-meshheading:1883485-Cloning, Molecular,
pubmed-meshheading:1883485-Cricetinae,
pubmed-meshheading:1883485-DNA,
pubmed-meshheading:1883485-Humans,
pubmed-meshheading:1883485-Insulin-Like Growth Factor I,
pubmed-meshheading:1883485-Molecular Sequence Data,
pubmed-meshheading:1883485-Peptide Fragments,
pubmed-meshheading:1883485-Recombinant Proteins,
pubmed-meshheading:1883485-Transfection
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|
pubmed:year |
1991
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|
pubmed:articleTitle |
Expression, purification and characterization of secreted recombinant human insulin-like growth factor-I (IGF-I) and the potent variant des(1-3) IGF-I in Chinese hamster ovary cells.
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pubmed:affiliation |
Department of Biochemistry, University of Adelaide, South Australia.
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pubmed:publicationType |
Journal Article
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