Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1991-10-2
pubmed:abstractText
A rat liver mitochondrial-matrix fraction was prepared and shown to have 1-chloro-2,4-dinitrobenzene(CDNB)-metabolizing glutathione transferase (GST) activity. Further fractionation by sequential gel filtration, isoelectric focusing or chromatofocusing and hydroxyapatite chromatography yielded three GSTs of pI 9.3, 8.9 and 7.5, none of which bound to a GSH-agarose affinity matrix. Most of the activity was associated with the pI-9.3 form, which was selected for further study. Its activity was tested with the following potential substrates in addition to CDNB: 1,2-dichloro-4-nitrobenzene, p-nitrobenzyl chloride, trans-4-phenylbut-3-en-2-one, 1,2-epoxy-3-(p-nitrophenoxy)propane, ethacrynic acid, menaphthyl sulphate, cumene hydroperoxide, linoleic acid hydroperoxide and 4-hydroxynon-2-enal. Appreciable activity was obtained only with CDNB and ethacrynic acid (82 and 26 mumol/min per mg of protein respectively). The apparent Km for GSH, using 1 mM-CDNB, was 1.9 mM. The enzyme is a dimer of subunit Mr 26,500. It has a free N-terminus, which has enabled the first 33 amino acids to be sequenced. This portion of primary structure has a sequence in common with members of the Theta class of GSTs (eg. 36% identity with subunit 12) and also a sequence which might function as a mitochondrial import signal. It is novel and has been named 'GST 13-13'.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-1003213, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-165008, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-1848757, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-2104602, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-2114406, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-2182291, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-2505855, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-2553668, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-2713846, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-2764905, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-3145740, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-3196296, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-3338112, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-3396537, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-3838159, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-384160, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-3864155, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-3994769, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-4044571, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-4371162, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-4418023, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-4436300, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-5058327, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-5472159, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-5787688, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-6436235, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-6439207, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-6477531, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-6653549, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-6770466, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-7061508, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-7173206, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-7219534, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-7358335, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-7432460, http://linkedlifedata.com/resource/pubmed/commentcorrection/1883325-942051
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
278 ( Pt 1)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
137-41
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
A novel glutathione transferase (13-13) isolated from the matrix of rat liver mitochondria having structural similarity to class theta enzymes.
pubmed:affiliation
Department of Biochemistry, University College, London, U.K.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't