|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0024267,
umls-concept:C0042769,
umls-concept:C0085358,
umls-concept:C0185027,
umls-concept:C0871261,
umls-concept:C1332714,
umls-concept:C1332717,
umls-concept:C1413244,
umls-concept:C1704632,
umls-concept:C1706438,
umls-concept:C1706817,
umls-concept:C2698600,
umls-concept:C2911692
|
|
pubmed:issue |
23
|
|
pubmed:dateCreated |
2008-11-12
|
|
pubmed:abstractText |
Activation of CD4(+) T cells helps establish and sustain other immune responses. We have previously shown that responses against a broad set of nine CD4(+) T-cell epitopes were present in the setting of lymphocytic choriomeningitis virus (LCMV) Armstrong infection in the context of H-2(d). This is quite disparate to the H-2(b) setting, where only two epitopes have been identified. We were interested in determining whether a broad set of responses was unique to H-2(d) or whether additional CD4(+) T-cell epitopes could be identified in the setting of the H-2(b) background. To pursue this question, we infected C57BL/6 mice with LCMV Armstrong and determined the repertoire of CD4(+) T-cell responses using overlapping 15-mer peptides corresponding to the LCMV Armstrong sequence. We confirmed positive responses by intracellular cytokine staining and major histocompatibility complex (MHC)-peptide binding assays. A broad repertoire of responses was identified, consisting of six epitopes. These epitopes originate from the nucleoprotein (NP) and glycoprotein (GP). Out of the six newly identified CD4(+) epitopes, four of them also stimulate CD8(+) T cells in a statistically significant manner. Furthermore, we assessed these CD4(+) T-cell responses during the memory phase of LCMV Armstrong infection and after infection with a chronic strain of LCMV and determined that a subset of the responses could be detected under these different conditions. This is the first example of a broad repertoire of shared epitopes between CD4(+) and CD8(+) T cells in the context of viral infection. These findings demonstrate that immunodominance is a complex phenomenon in the context of helper responses.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-10400769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-10500077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-10639312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-10716904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-10852914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-11186304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-11551929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-11606739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-11987822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-12496434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-12574376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-12594515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-12663797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-12690201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-14607945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-15034033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-15078175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-15078927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-15140950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-15245741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-15483230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-15749848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-15939787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-16051844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-16140434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-16237051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-16364742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-16382236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-16893568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-16901572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-16921384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-17000870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-17151096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-17320138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-17329346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-17507491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-17513729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-17542751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-17617598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-2448497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-2477254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-2705303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-7533855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-7911534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-7966595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-8469287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-8566030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-9064346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-9448700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-9485218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-9806631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-9808199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829752-9858507
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
1098-5514
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
82
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
11734-41
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
|
