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rdf:type |
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lifeskim:mentions |
umls-concept:C0085358,
umls-concept:C0280100,
umls-concept:C0598934,
umls-concept:C0871261,
umls-concept:C1332717,
umls-concept:C1413244,
umls-concept:C1704632,
umls-concept:C1706438,
umls-concept:C1706817,
umls-concept:C2698600,
umls-concept:C2911692
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pubmed:issue |
19
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pubmed:dateCreated |
2008-10-2
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pubmed:abstractText |
Bone marrow-derived dendritic cells engineered using recombinant adenovirus to secrete high levels of IL-12p70 dramatically inhibited the growth of established CMS4 sarcomas in BALB/c mice after intratumoral administration. An analysis of splenic CD8(+) T cells in regressor mice revealed a strong, complex reactivity pattern against high-performance liquid chromatography (HPLC)-resolved peptides isolated by acid elution from single-cell suspensions of surgically resected CMS4 lesions. Mass spectrometry analyses defined two major overlapping peptide species that derive from the murine hemoglobin-beta (HBB) protein within the most stimulatory HPLC fractions. Although cultured CMS4 tumor cells failed to express HBB mRNA based on reverse transcription-PCR analyses, prophylactic vaccination of BALB/c mice with vaccines containing HBB peptides promoted specific CD8(+) T-cell responses that protected mice against a subsequent challenge with CMS4 or unrelated syngeneic (HBB(neg)) tumors of divergent histology (sarcoma, carcinomas of the breast or colon). In situ imaging suggested that vaccines limit or destabilize tumor-associated vascular structures, potentially by promoting immunity against HBB+ vascular pericytes. Importantly, there were no untoward effects of vaccination with the HBB peptide on peripheral RBC numbers, RBC hemoglobin content, or vascular structures in the brain or eye.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-10359765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-10518571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-10844666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-10954746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-12351455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-14559827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-15069689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-15169764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-15478309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-15661540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-15730849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-15930316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-15979582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-16164022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-16212810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-16303125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-16357940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-16365598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-16651446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-16778141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-16819514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-17020992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-17050049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-17125590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-17363608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-17916598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-17978043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-18276779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-3651609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-7259087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-7690811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-9211853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-9224969,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-9603645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-9950880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18829566-9973316
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1538-7445
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8076-84
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:18829566-Animals,
pubmed-meshheading:18829566-CD8-Positive T-Lymphocytes,
pubmed-meshheading:18829566-Cancer Vaccines,
pubmed-meshheading:18829566-Cell Proliferation,
pubmed-meshheading:18829566-Dendritic Cells,
pubmed-meshheading:18829566-Female,
pubmed-meshheading:18829566-Hemoglobins,
pubmed-meshheading:18829566-Immunity, Cellular,
pubmed-meshheading:18829566-Immunotherapy, Adoptive,
pubmed-meshheading:18829566-Mice,
pubmed-meshheading:18829566-Mice, Inbred BALB C,
pubmed-meshheading:18829566-Neoplasms,
pubmed-meshheading:18829566-Peptide Fragments,
pubmed-meshheading:18829566-Transplantation, Isogeneic,
pubmed-meshheading:18829566-Tumor Burden,
pubmed-meshheading:18829566-Tumor Cells, Cultured
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pubmed:year |
2008
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pubmed:articleTitle |
CD8+ T-cell responses against hemoglobin-beta prevent solid tumor growth.
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pubmed:affiliation |
Department of Dermatology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA.
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