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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2008-11-17
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pubmed:abstractText |
A transient increase in intracellular Ca(2+) is the universal signal for egg activation at fertilization. Eggs acquire the ability to mount the specialized fertilization-specific Ca(2+) signal during oocyte maturation. The first Ca(2+) transient following sperm entry in vertebrate eggs has a slow rising phase followed by a sustained plateau. The molecular determinants of the sustained plateau are poorly understood. We have recently shown that a critical determinant of Ca(2+) signaling differentiation during oocyte maturation is internalization of the plasma membrane calcium ATPase (PMCA). PMCA internalization is representative of endocytosis of several integral membrane proteins during oocyte maturation, a requisite process for early embryogenesis. Here we investigate the mechanisms regulating PMCA internalization. To track PMCA trafficking in live cells we cloned a full-length cDNA of Xenopus PMCA1, and show that GFP-tagged PMCA traffics in a similar fashion to endogenous PMCA. Functional data show that MPF activation during oocyte maturation is required for full PMCA internalization. Pharmacological and co-localization studies argue that PMCA is internalized through a lipid raft endocytic pathway. Deletion analysis reveal a requirement for the N-terminal cytoplasmic domain for efficient internalization. Together these studies define the mechanistic requirements for PMCA internalization during oocyte maturation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-10359788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-10395780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-10991950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-11063930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-11473540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-11707525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-11781335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-12651740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-1377448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-1385064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-1480112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-15067021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-15136768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-15375168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-15464580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-15668297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-15848178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-16102821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-16126729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-16249176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-16330019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-17596212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-17620315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-17699605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-17804821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-17956246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-18029012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-1879345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-2040371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-2154110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-2390997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-3980584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-4008532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-6088774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-6682118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-6736888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-7070509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-7559755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-7626797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-7690240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-8288861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-8382206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-9315885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-9395767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823969-9585411
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1095-564X
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
324
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
99-107
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pubmed:dateRevised |
2010-9-21
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pubmed:meshHeading |
pubmed-meshheading:18823969-Amino Acid Sequence,
pubmed-meshheading:18823969-Animals,
pubmed-meshheading:18823969-Calcium Signaling,
pubmed-meshheading:18823969-Cell Membrane,
pubmed-meshheading:18823969-Endocytosis,
pubmed-meshheading:18823969-Female,
pubmed-meshheading:18823969-Molecular Sequence Data,
pubmed-meshheading:18823969-Oocytes,
pubmed-meshheading:18823969-Oogenesis,
pubmed-meshheading:18823969-Plasma Membrane Calcium-Transporting ATPases,
pubmed-meshheading:18823969-Protein Structure, Tertiary,
pubmed-meshheading:18823969-Protein Transport,
pubmed-meshheading:18823969-Xenopus Proteins,
pubmed-meshheading:18823969-Xenopus laevis
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pubmed:year |
2008
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pubmed:articleTitle |
Internalization of plasma membrane Ca2+-ATPase during Xenopus oocyte maturation.
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pubmed:affiliation |
Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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