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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2008-10-22
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pubmed:abstractText |
c-JUN is a major component of heterodimer transcription factor AP-1 (Activator Protein-1) that activates gene transcription in cell proliferation, inflammation and stress responses. SIRT1 (Sirtuin 1) is a histone deacetylase that controls gene transcription through modification of chromatin structure. However, it is not clear if SIRT1 regulates c-JUN activity in the control of gene transcription. Here, we show that SIRT1 associated with c-JUN in co-immunoprecipitation of whole cell lysate, and inhibited the transcriptional activity of c-JUN in the mammalian two hybridization system. SIRT1 was found in the AP-1 response element in the matrix metalloproteinase-9 (MMP9) promoter DNA leading to inhibition of histone 3 acetylation as shown in a ChIP assay. The SIRT1 signal was reduced by the AP-1 activator PMA, and induced by the SIRT1 activator Resveratrol in the promoter DNA. SIRT1-mediaetd inhibition of AP-1 was demonstrated in the MMP9 gene expression at the gene promoter, mRNA and protein levels. In mouse embryonic fibroblast (MEF) with SIRT1 deficiency (SIRT1(-/-)), mRNA and protein of MMP9 were increased in the basal condition, and the inhibitory activity of Resveratrol was significantly attenuated. Glucose-induced MMP9 expression was also inhibited by SIRT1 in response to Resveratrol. These data consistently suggest that SIRT1 directly inhibits the transcriptional activity of AP-1 by targeting c-JUN.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823944-10935492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823944-11087751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823944-12213555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823944-12351658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823944-12939617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823944-12960381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823944-14661062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823944-15213027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823944-15269222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823944-15277439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823944-15604409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823944-15744310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823944-15811852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823944-16150801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823944-16183991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823944-16246065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823944-17908551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823944-18046409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823944-18423418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823944-18577374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823944-8631874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823944-9115218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823944-9590175
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1090-2104
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
28
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pubmed:volume |
376
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
793-6
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pubmed:dateRevised |
2011-5-2
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pubmed:meshHeading |
pubmed-meshheading:18823944-Animals,
pubmed-meshheading:18823944-Antioxidants,
pubmed-meshheading:18823944-Cell Line,
pubmed-meshheading:18823944-Female,
pubmed-meshheading:18823944-Gene Expression Regulation,
pubmed-meshheading:18823944-Humans,
pubmed-meshheading:18823944-Immunoprecipitation,
pubmed-meshheading:18823944-Male,
pubmed-meshheading:18823944-Matrix Metalloproteinase 9,
pubmed-meshheading:18823944-Mice,
pubmed-meshheading:18823944-Mice, Knockout,
pubmed-meshheading:18823944-Proto-Oncogene Proteins c-jun,
pubmed-meshheading:18823944-Sirtuin 1,
pubmed-meshheading:18823944-Sirtuins,
pubmed-meshheading:18823944-Stilbenes,
pubmed-meshheading:18823944-Transcription, Genetic,
pubmed-meshheading:18823944-Two-Hybrid System Techniques
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pubmed:year |
2008
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pubmed:articleTitle |
Inhibition of transcriptional activity of c-JUN by SIRT1.
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pubmed:affiliation |
Pennington Biomedical Research Center, Louisiana State University System, 6400 Perkins Road, Baton Rouge, LA 70808, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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