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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2008-10-24
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pubmed:abstractText |
Vibrio parahaemolyticus is a marine seafood-borne pathogen causing gastrointestinal disorders in humans. Thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) are known as major virulence determinants of V. parahaemolyticus. Most V. parahaemolyticus isolates from the environment do not produce TDH or TRH. Total V. parahaemolyticus has been used as an indicator for control of seafood contamination toward prevention of infection. Detection of total V. parahaemolyticus using conventional culture- and biochemical-based assays is time-consuming and laborious, requiring more than three days. Thus, we developed a novel and highly specific loop-mediated isothermal amplification (LAMP) assay for the sensitive and rapid detection of Vibrio parahaemolyticus.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823567-10379807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823567-10871386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823567-12144774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823567-12791888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823567-1480187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823567-16242860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823567-16517652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823567-17088368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823567-17314373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823567-17644647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823567-18158196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823567-18349363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823567-18547441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18823567-3508495
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1471-2180
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
163
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2008
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pubmed:articleTitle |
Development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of Vibrio parahaemolyticus.
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pubmed:affiliation |
Division of Bacteriology, Osaka Prefectural Institute of Public Health, Osaka, Japan. wataru@iph.pref.osaka.jp
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pubmed:publicationType |
Journal Article
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