Linked Life Data

18823074

Source:http://linkedlifedata.com/resource/pubmed/id/18823074

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-1-15
pubmed:abstractText
Two methods are presented for the determination of 'respectively' the plasma protein unbound and total concentration of acyclovir in horse plasma and body fluids: first, a liquid-liquid extraction was performed on plasma, combined with HPLC-fluorescence detection for the total plasma concentration; second a more sensitive method using high-performance liquid chromatography combined with heated electrospray ionization tandem mass spectrometry (LC-HESI-MS/MS) was described for plasma and for body fluids analysis. To obtain the unbound concentration of acyclovir in plasma, a simple deproteinization step using a Microcon filter was performed. Ganciclovir was used as an internal standard. Analysis was carried out on an Inertsil 5 ODS-3 column for the HPLC-fluorescence method. For the LC-HESI-MS/MS method a PLRP-S column was used. The limit of quantification (LOQ) for the total concentration was set at 50 and 2 ng mL(-1) for the HPLC-fluorescence method and the LC-HESI-MS/MS method, respectively. The limit of quantification for the unbound concentration was set at 5 ng mL(-1) and at 2 ng mL(-1) for body fluids. The methods were successfully used to perform pharmacokinetic and clinical studies in horses after intravenous and oral dosage of acyclovir and its prodrug valacyclovir.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1099-0801
pubmed:author
pubmed:copyrightInfo
Copyright (c) 2008 John Wiley & Sons, Ltd.
pubmed:issnType
Electronic
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
132-40
pubmed:dateRevised
2009-7-17
pubmed:meshHeading
pubmed-meshheading:18823074-Acyclovir, pubmed-meshheading:18823074-Administration, Oral, pubmed-meshheading:18823074-Animals, pubmed-meshheading:18823074-Antiviral Agents, pubmed-meshheading:18823074-Body Fluids, pubmed-meshheading:18823074-Chromatography, High Pressure Liquid, pubmed-meshheading:18823074-Fluorescence, pubmed-meshheading:18823074-Ganciclovir, pubmed-meshheading:18823074-Horses, pubmed-meshheading:18823074-Infusions, Intravenous, pubmed-meshheading:18823074-Linear Models, pubmed-meshheading:18823074-Reference Standards, pubmed-meshheading:18823074-Reproducibility of Results, pubmed-meshheading:18823074-Sensitivity and Specificity, pubmed-meshheading:18823074-Spectrometry, Mass, Electrospray Ionization, pubmed-meshheading:18823074-Tandem Mass Spectrometry, pubmed-meshheading:18823074-Valine
pubmed:year
2009
pubmed:articleTitle
Determination of acyclovir in horse plasma and body fluids by high-performance liquid chromatography combined with fluorescence detection and heated electrospray ionization tandem mass spectrometry.
pubmed:affiliation
Department of Pharmacology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. an.maes@ugent.be
pubmed:publicationType
Journal Article, Validation Studies