Source:http://linkedlifedata.com/resource/pubmed/id/18820661
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2008-12-31
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| pubmed:abstractText |
Gastric extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue (MZL-MALT) is speculated to be immune mediated and is notable for responding to treatment by Helicobacter pylori eradication. However, the gastric MZL-MALT with t(11;18)(q21;q21) has been shown to be resistant to treatment by H. pylori eradication. We studied the molecular, immunohistochemical, and histological aspects of 48 cases of gastric MZL-MALT and used a reverse transcription real-time PCR assay to assess the presence of a t(11;18)(q21;q21) in formalin-fixed, paraffin-embedded tissue. Florescence in situ hybridization for t(11:18)(q21;q21) was used to confirm the real-time PCR results. Three distinct morphological subtypes were recognized: monocytoid, small lymphocytic, and plasmacytoid. Morphology, immunophenotype, and immunoglobulin heavy chain (IgH) gene rearrangement were correlated with the results of the t(11:18)(q21;q21) assay. Of the 48 analyzed cases, 15 (31%) were positive for t(11;18)(q21;q21) and 33 (69%) were monoclonal for IgH gene rearrangement. Of the 15, 13 (87%) cases with t(11;18)(q21;q21) translocation showed IgH gene rearrangement by PCR. Of the 33 t(11;18)(q21;q21)-negative cases tested, 20 cases (61%) showed IgH gene rearrangement. The 15 t(11;18)(q21;q21) translocation-positive cases had either monocytoid (12 of 15) or small lymphocytic morphology (3 of 15). Aberrant expression of CD43 was observed in 8 of 15 (53%) t(11;18)(q21;q21)-positive cases and 21 of 31 (68%) t(11;18)(q21;q21)-negative cases. Our data show that t(11;18)(q21;q21)-positive MZL-MALTs frequently show monocytoid morphology, less often small lymphocytic morphology, and not purely plasmacytoid morphology. Identification of a t(11;18)(q21;q21) by reverse transcription real-time PCR is highly specific for MZL-MALT and helps in the diagnosis of MZL-MALT. Studying the correlation between this translocation and morphological features may increase our understanding of the role of this translocation in the pathogenesis and the clinical behavior of gastric MZL-MALT.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
1530-0285
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
22
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
79-86
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| pubmed:meshHeading |
pubmed-meshheading:18820661-Adult,
pubmed-meshheading:18820661-Aged,
pubmed-meshheading:18820661-Aged, 80 and over,
pubmed-meshheading:18820661-Antigens, CD43,
pubmed-meshheading:18820661-Chromosomes, Human, Pair 11,
pubmed-meshheading:18820661-Chromosomes, Human, Pair 18,
pubmed-meshheading:18820661-Female,
pubmed-meshheading:18820661-Gene Rearrangement, B-Lymphocyte, Heavy Chain,
pubmed-meshheading:18820661-Humans,
pubmed-meshheading:18820661-Immunohistochemistry,
pubmed-meshheading:18820661-In Situ Hybridization, Fluorescence,
pubmed-meshheading:18820661-Lymphoma, B-Cell, Marginal Zone,
pubmed-meshheading:18820661-Male,
pubmed-meshheading:18820661-Middle Aged,
pubmed-meshheading:18820661-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:18820661-Stomach Neoplasms,
pubmed-meshheading:18820661-Translocation, Genetic
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| pubmed:year |
2009
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| pubmed:articleTitle |
t(11;18)(q21;q21) in extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue in stomach: a study of 48 cases.
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| pubmed:affiliation |
Division of Molecular Pathology, Department of Scientific Laboratories, Armed Forces Institute of Pathology, Washington DC 20306-6000, USA. guanghua.wang@afip.osd.mil
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| pubmed:publicationType |
Journal Article
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