Linked Life Data

18817322

Source:http://linkedlifedata.com/resource/pubmed/id/18817322

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-9-26
pubmed:abstractText
Toll-like receptor 4 (TLR4) is the key receptor for the LPS component of Gram-negative bacteria. We have identified a SNP within the 5' UTR region of the bovine TLR4 gene at position 226 (c.-226C>G) relative to start codon, which was found to be associated with health related traits in the Canadian Holstein population. TLR4 gene expression was measured over time intervals in samples collected from 18 cows (6 cows in each genotype) challenged in vitro with LPS (20 ng/ml) to determine if this SNP alters the gene expression. TLR4 gene expression was highest 6 h post LPS challenge, and cows with the GG genotype exhibited significantly higher expression compared to other genotypes. This was further validated by a dual luciferase reporter gene assay in which sequential progressive constructs were cloned into the pGL3 basic vector and transfected into bovine Mac-T cells. The reporter construct containing most of the 5' UTR (-456G) exhibited significantly higher luciferase activity compared to-456C, however, further inclusion of 5' upstream sequence showed a reversal phenomenon where the -913C construct exhibited significantly higher activity compared to -913G. These results indicate that the c.-226C>G SNP affects the expression of TLR4, suggesting that this SNP might be influencing the binding and interaction of transcription factors regulating gene expression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1424-6074
pubmed:author
pubmed:issnType
Print
pubmed:volume
132
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
331-6
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Functional characterization of a single nucleotide polymorphism in the 5' UTR region of the bovine toll-like receptor 4 gene.
pubmed:affiliation
Centre for the Genetic Improvement of Livestock, Department of Animal and Poultry Science, University of Guelph, Guelph, Canada.
pubmed:publicationType
Journal Article