Linked Life Data

18816824

Source:http://linkedlifedata.com/resource/pubmed/id/18816824

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2008-10-2
pubmed:abstractText
In this communication, we report the generation of a cre transgenic zebrafish line under an oocyte-specific promoter, zp3. The transgenic line Tg(zp3:cre; krt8:rfp) also contains a co-integrated rfp transgene under the skin epithelial promoter krt8 to allow selection of cre transgenic fish based on RFP fluorescence in the skin. We demonstrated in this transgenic line that cre mRNA was specifically expressed in growing oocytes like endogenous zp3 mRNA. When Tg(zp3:cre; krt8:rfp) was crossed with a loxP transgenic line, the floxed DNA was specifically eliminated from female, but not male, germline. Tg(zp3:cre; krt8:rfp) fish also have maternal cre mRNA in early embryos to cause Cre-mediated recombination; this feature can be used to activate other loxP transgenic lines in early embryos. Furthermore, after crossing with another loxP transgenic line, Tg(EF:loxP-mCherry-loxP-egfp), we confirmed that our cre line was capable of activating a loxP-blocked EGFP reporter gene by both maternal and oocyte-expressed Cre.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1058-8388
pubmed:author
pubmed:copyrightInfo
Copyright (c) 2008 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
237
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2955-62
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Generation of oocyte-specifically expressed cre transgenic zebrafish for female germline excision of loxP-flanked transgene.
pubmed:affiliation
Department of Biological Sciences, National University of Singapore, Singapore.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't