Source:http://linkedlifedata.com/resource/pubmed/id/18816083
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
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pubmed:dateCreated |
2008-11-10
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pubmed:abstractText |
There is a correlation between the multidrug-resistance (MDR) of cancer cells and their enhanced invasive or metastatic potential. We studied the expression of CD147, a plasma membrane glycoprotein that plays a key role in tumor metastasis by stimulating the production of matrix metalloproteinases (MMPs), in sensitive human oral squamous KB and MDR derivative KB/V cells. Reverse transcription-PCR and flow cytometric analysis revealed that KB/V cells expressed CD147 at significantly higher levels than their parental KB cells. Using stable RNA interference, we succeeded in establishing a CD147 knock-down KB/V cell line (KB/VsiCD147). MTT colorimetric assay showed an increase in the chemosensitivity to vincristine (VCR), all transretinoic acid (ATRA), taxol, and 5-fluorouracil (5-Fu) of KB/VsiCD147 cells. Proteome analysis of KB, KB/V, and KB/VsiCD147 cell lines identified 21 differently expressed proteins. The enhanced expression of representative active proteins, GRP75 and CyPA, was confirmed by Western blotting and RT-PCR. In addition, pretreatment of KB/V cells with a CyPA-binding immunosuppressive drug, cyclosporine A (CsA), enhanced their chemosensitivity to VCR and 5-Fu. We document an abundance of molecules that interact with CD147 in the MDR of human oral squamous carcinoma cells. Additional studies are needed to investigate these novel target proteins of CD147.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD147,
http://linkedlifedata.com/resource/pubmed/chemical/Coloring Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Formazans,
http://linkedlifedata.com/resource/pubmed/chemical/MTT formazan,
http://linkedlifedata.com/resource/pubmed/chemical/Proteome,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrazolium Salts
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1535-3893
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4784-91
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pubmed:meshHeading |
pubmed-meshheading:18816083-Antigens, CD147,
pubmed-meshheading:18816083-Carcinoma, Squamous Cell,
pubmed-meshheading:18816083-Cell Line, Tumor,
pubmed-meshheading:18816083-Coloring Agents,
pubmed-meshheading:18816083-Drug Resistance, Multiple,
pubmed-meshheading:18816083-Drug Resistance, Neoplasm,
pubmed-meshheading:18816083-Formazans,
pubmed-meshheading:18816083-Humans,
pubmed-meshheading:18816083-Proteome,
pubmed-meshheading:18816083-Proteomics,
pubmed-meshheading:18816083-RNA, Small Interfering,
pubmed-meshheading:18816083-RNA Interference,
pubmed-meshheading:18816083-Tetrazolium Salts,
pubmed-meshheading:18816083-Transfection
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pubmed:year |
2008
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pubmed:articleTitle |
Proteome analysis of multidrug resistance of human oral squamous carcinoma cells using CD147 silencing.
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pubmed:affiliation |
Department of Dermatology, Xiang Ya Hospital, Central South University, Hunan, 410008, China, Department of Dermatology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, 890-8520, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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