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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2008-11-21
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pubmed:abstractText |
Controlled modulation of T-cell response during immunotherapy, especially the balance between T helper 1 (Th1) and Th2 responses, is critical for generating effective immune response. Here we report that dual delivery of interleukin 10 (IL-10)-targeted small interfering RNA (siRNA) and DNA vaccines to dendritic cells (DCs), using a single particle carrier, efficiently enhances immune response and modulates it toward a stronger Th1 phenotype. Surface-functionalized polymer microparticles (MPs) carrying both IL-10-targeted siRNA and DNA antigens exhibited effective gene silencing, DNA transfection, and synergistically enhanced upregulation of maturation markers in primary DCs leading to increased T-cell proliferation, in vitro. Mice immunized with these dual-delivery carriers demonstrated a significant "switch" toward Th1 response as evidenced by increase in interferon gamma (IFN-gamma) production and decrease in IL-4 production by CD4+ T cells. This further led to enhanced antiviral cytotoxic T-lymphocyte activity. Such dual siRNA-DNA delivery provides a novel strategy to precisely control the type and strength of T-cell response during immunotherapy.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Imines,
http://linkedlifedata.com/resource/pubmed/chemical/Lactic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Polyethylenes,
http://linkedlifedata.com/resource/pubmed/chemical/Polyglycolic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, DNA,
http://linkedlifedata.com/resource/pubmed/chemical/poly(ethylene imine),
http://linkedlifedata.com/resource/pubmed/chemical/polylactic acid-polyglycolic acid...
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1525-0024
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2011-21
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pubmed:meshHeading |
pubmed-meshheading:18813280-Animals,
pubmed-meshheading:18813280-Antigen-Presenting Cells,
pubmed-meshheading:18813280-Biological Markers,
pubmed-meshheading:18813280-Bone Marrow,
pubmed-meshheading:18813280-Cell Proliferation,
pubmed-meshheading:18813280-Cells, Cultured,
pubmed-meshheading:18813280-Cytokines,
pubmed-meshheading:18813280-Dendritic Cells,
pubmed-meshheading:18813280-Female,
pubmed-meshheading:18813280-Gene Expression Regulation,
pubmed-meshheading:18813280-Imines,
pubmed-meshheading:18813280-Lactic Acid,
pubmed-meshheading:18813280-Mice,
pubmed-meshheading:18813280-Mice, Inbred BALB C,
pubmed-meshheading:18813280-Plasmids,
pubmed-meshheading:18813280-Polyethylenes,
pubmed-meshheading:18813280-Polyglycolic Acid,
pubmed-meshheading:18813280-RNA, Small Interfering,
pubmed-meshheading:18813280-T-Lymphocytes,
pubmed-meshheading:18813280-Up-Regulation,
pubmed-meshheading:18813280-Vaccines, DNA
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pubmed:year |
2008
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pubmed:articleTitle |
Efficient modulation of T-cell response by dual-mode, single-carrier delivery of cytokine-targeted siRNA and DNA vaccine to antigen-presenting cells.
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pubmed:affiliation |
Department of Biomedical Engineering, The University of Texas at Austin, Austin, Texas 78712, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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