Source:http://linkedlifedata.com/resource/pubmed/id/18810860
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
2008-9-24
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| pubmed:abstractText |
Recent work from our laboratory revealed that tryptic soy broth (TSB) was a superior enrichment medium for use in test-and-hold Escherichia coli O157:H7 methods at levels down to a ratio of three volumes of medium to one volume of sample. Lower ratios were examined for their effect on results obtained from culture isolation, the BAX E. coli O157:H7 MP assay, and the Assurance GDS E. coli O157:H7 assay. Ground beef and boneless beef trim were inoculated with a high level (170 CFU/65 g of ground beef and 43 CFU/65 g of trim) and a low level (17 CFU/65 g of ground beef and 4 CFU/65 g of trim) of E. coli O157:H7 and enriched in 3, 1, 0.5, and 0 volumes of TSB. The volume of TSB used did not affect E. coli O157:H7 detection by culture isolation, Assurance GDS detection in ground beef or trim, or the BAX MP assay detection in ground beef. However, BAX MP assay detection of E. coli O157:H7 in beef trim was 50, 42, and 33% positive when enrichment volumes of 0.5x, 1x, and 3x, respectively, were used. Optimum results with all methods were obtained using 1 volume of TSB. We concluded that detection test results can be considered valid as long as enrichment medium is used, even when it is less than the specified 3 or 10 volumes.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
0362-028X
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
71
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1768-73
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| pubmed:meshHeading |
pubmed-meshheading:18810860-Animals,
pubmed-meshheading:18810860-Cattle,
pubmed-meshheading:18810860-Colony Count, Microbial,
pubmed-meshheading:18810860-Consumer Product Safety,
pubmed-meshheading:18810860-Culture Media,
pubmed-meshheading:18810860-Escherichia coli O157,
pubmed-meshheading:18810860-Food Contamination,
pubmed-meshheading:18810860-Humans,
pubmed-meshheading:18810860-Meat,
pubmed-meshheading:18810860-Meat Products
|
| pubmed:year |
2008
|
| pubmed:articleTitle |
Effects of using reduced volumes of nonselective enrichment medium in methods for the detection of Escherichia coli O157:H7 from raw beef.
|
| pubmed:affiliation |
U.S. Department of Agriculture, Agricultural Research Service, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, Nebraska 68933-0166, USA. mick.bosilevac@ars.usda.gov
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| pubmed:publicationType |
Journal Article
|