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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2008-11-18
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pubmed:abstractText |
The role of CD4(+) T-cell interleukin-4 (IL-4) receptor alpha (IL-4Ralpha) expression in T helper 2 (TH2) immune responses has not been defined. To examine this role, we infected CD4(+) T-cell IL-4Ralpha knockout (KO) mice with the parasitic nematode Nippostrongylus brasiliensis, which induces strong host TH2 responses. Although N. brasiliensis expulsion was not affected in CD4(+) T-cell IL-4Ralpha KO mice, the associated lung pathology was reduced. Infected CD4(+) T-cell IL-4Ralpha KO mice showed abrogation of airway mucus production. Furthermore, CD4(+) T-cell IL-4Ralpha KO mouse lungs contained reduced numbers of lymphocytes and eosinophils. Restimulation of pulmonary region-associated T-cell populations showed that TH2 cytokine responses were disrupted. Secretion of IL-4, but not secretion of IL-13 or IL-5, from mediastinal lymph node CD4(+) T cells was reduced in infected CD4(+) T-cell IL-4Ralpha KO mice. Restimulation of tissue-derived CD4(+) T cells resulted in equivalent levels of IL-4 and IL-13 on day 7 postinfection (p.i.) in control and CD4(+) T-cell IL-4Ralpha KO mice. By day 10 p.i. the TH2 cytokine levels had significantly declined in CD4(+) T-cell IL-4Ralpha KO mice. Restimulation with N. brasiliensis antigen of total lung cell populations and populations with CD4(+) T cells depleted showed that CD4(+) T cells were a key TH2 cytokine source. These data demonstrated that CD4(+) T-cell IL-4 responsiveness facilitates eosinophil and lymphocyte recruitment, lymphocyte localization, and TH2 cytokine production in the allergic pathology associated with N. brasiliensis infections.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-10229862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-10358179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-10358772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-10586888,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-11804871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-11856442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-12356576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-12397019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-12847266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-14635046,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-17222057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-17500591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-17709489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-18203142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-1869831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-2062833,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-3486902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-3794288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-3932582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-7907877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-8179909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-8294877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-8384701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-8468481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-9362533,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-9380721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-9492006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-9512421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-9635196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18809669-9780144
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1098-5522
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
76
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5535-42
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:18809669-Animals,
pubmed-meshheading:18809669-Chemotaxis, Leukocyte,
pubmed-meshheading:18809669-Cytokines,
pubmed-meshheading:18809669-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:18809669-Flow Cytometry,
pubmed-meshheading:18809669-Immunohistochemistry,
pubmed-meshheading:18809669-Interleukin-4,
pubmed-meshheading:18809669-Lung,
pubmed-meshheading:18809669-Mice,
pubmed-meshheading:18809669-Mice, Knockout,
pubmed-meshheading:18809669-Nippostrongylus,
pubmed-meshheading:18809669-Strongylida Infections,
pubmed-meshheading:18809669-Th2 Cells
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pubmed:year |
2008
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pubmed:articleTitle |
Interleukin-4-promoted T helper 2 responses enhance Nippostrongylus brasiliensis-induced pulmonary pathology.
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pubmed:affiliation |
International Centre for Genetic Engineering and Biotechnology, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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