Linked Life Data

18807018

Source:http://linkedlifedata.com/resource/pubmed/id/18807018

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-12-18
pubmed:abstractText
Studies into the interactions between drugs and human serum albumin (HSA) are extremely important for drug discovery, since HSA behaves as a carrier for external drugs and internal biological molecules. In this paper, to evaluate the pharmacokinetic and pharmacodynamic properties of dexamethasone (DXM), the interaction between DXM and HSA was studied by capillary electrophoresis-frontal analysis (CE-FA). According to the Klotz equation, four binding sites between DXM and HSA were obtained, and the average binding constant was 1.05 x 10(3) M(-1). Furthermore, according to multiple equilibrium theory, based on the assumption that there are two types of binding site, the binding constant at one site was calculated to be 3.539 x 10(3) M(-1), and the average of the other three was 1.234 x 10(3) M(-1). In addition, to obtain the detailed binding information at each binding site, new equations were deduced by multivariate regression. The four binding constants of DXM and HSA were calculated to be 5.558 x 10(1) M(-1), 2.158 x 10(4) M(-1), 7.312 x 10(3) M(-1) and 2.043 x 10(3) M(-1), respectively, which is helpful for detailed studies into the interactions between drugs and proteins with multiple binding sites.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1618-2650
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
393
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
257-61
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Study of multiple binding constants of dexamethasone with human serum albumin by capillary electrophoresis-frontal analysis and multivariate regression.
pubmed:affiliation
National Chromatographic Research and Analysis Center, Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian, 116023, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't