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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1991-9-27
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pubmed:abstractText |
We developed and optimized an enzyme immunoassay for human neutrophil defensins, cationic cysteine-rich peptides that participate in host defense and inflammation. The assay utilizes a sandwich design with a monoclonal capture antibody and a biotinylated monoclonal detecting antibody. Cetrimonium bromide is employed to obviate non-specific binding of defensins to surfaces. The assay has a sensitivity of 0.04-0.05 ng/ml and a working range of 0.05-10 ng/ml.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-1759
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
9
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pubmed:volume |
141
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
149-55
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1880422-Antibodies, Monoclonal,
pubmed-meshheading:1880422-Blood Bactericidal Activity,
pubmed-meshheading:1880422-Blood Proteins,
pubmed-meshheading:1880422-Defensins,
pubmed-meshheading:1880422-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1880422-Humans,
pubmed-meshheading:1880422-Hybridomas,
pubmed-meshheading:1880422-Neutrophils
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pubmed:year |
1991
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pubmed:articleTitle |
An enzyme immunoassay for human defensins.
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pubmed:affiliation |
Will Rogers Pulmonary Research Laboratory, School of Medicine, University of California, Los Angeles 90024-1736.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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