Source:http://linkedlifedata.com/resource/pubmed/id/18804056
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
2008-9-22
|
pubmed:abstractText |
The effects of CoCl(2) on retinoic acid (RA)-treated embryoid bodies (EBs) were investigated. Four-day EBs were treated with 5x10(-6) M of RA for 4 d, then subjected to attached culturing for 7 d in the presence of CoCl(2) at 0, 20, and 100 microM. Differentiation into MAP2- and GFAP-immunopositive cells was inhibited by CoCl(2) in a dose-dependent manner. Next, RA-treated EBs were dissociated into single cells and cultured for 7 d at an initial cell density of 1x10(3)/cm(2). The number of cells increased in a CoCl(2)-dose dependent fashion. In cultures with 100 microM of CoCl(2), more than 90% of the cells were immunopositive for nestin and nestin-immunopositive cells formed clusters, while there were few cells immunopositive for MAP2 or GFAP. These results suggest that CoCl(2) inhibits neural differentiation of RA-treated EB cells and promotes the proliferation of nestin-immunopositive cells, i.e., embryonic stem (ES)-derived neural stem-like cells.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Aug
|
pubmed:issn |
1347-4421
|
pubmed:author |
pubmed-author:IshizakaShigeakiS,
pubmed-author:MatsudaRyosukeR,
pubmed-author:MoriyaKeiK,
pubmed-author:NakaseHiroyukiH,
pubmed-author:NishimuraFumihikoF,
pubmed-author:NishiofukuMarikoM,
pubmed-author:NonakaJun-ichiJ,
pubmed-author:OujiYukiteruY,
pubmed-author:SakakiToshisukeT,
pubmed-author:YamadaShuichiS,
pubmed-author:YoshikawaMasahideM
|
pubmed:issnType |
Electronic
|
pubmed:volume |
106
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
141-7
|
pubmed:meshHeading |
pubmed-meshheading:18804056-Animals,
pubmed-meshheading:18804056-Base Sequence,
pubmed-meshheading:18804056-Cell Differentiation,
pubmed-meshheading:18804056-Cell Proliferation,
pubmed-meshheading:18804056-Cobalt,
pubmed-meshheading:18804056-DNA Primers,
pubmed-meshheading:18804056-Dose-Response Relationship, Drug,
pubmed-meshheading:18804056-Embryonic Stem Cells,
pubmed-meshheading:18804056-Immunohistochemistry,
pubmed-meshheading:18804056-Neurons,
pubmed-meshheading:18804056-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:18804056-Tretinoin
|
pubmed:year |
2008
|
pubmed:articleTitle |
CoCl(2) inhibits neural differentiation of retinoic acid-treated embryoid bodies.
|
pubmed:affiliation |
Department of Neurosurgery, Nara Medical University, 840 Shijo-cho, Kashihara, Nara, Japan.
|
pubmed:publicationType |
Journal Article
|