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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1991-10-2
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pubmed:abstractText |
Responses to interleukin-2 (IL-2) of high-density human tonsillar B lymphocytes were examined in 20 microliters hanging drop microcultures. DNA synthesis and secretion of IgM and IgG were induced by IL-2 alone. Activation of calcium-dependent protein kinase C (PKC) with phorbol 12,13-dibutyrate and ionomycin increased IL-2 driven DNA synthesis yet reduced IL-2 driven secretion of IgM and IgG. Forskolin, which increases cAMP, had no effect on the responses to IL-2. Intrinsic IL-6 played no role in IL-2-induced DNA synthesis but was partially responsible for the secretion of immunoglobulin. These data show that pre-activation of the high-density human tonsillar B lymphocyte is not a prerequisite for IL-2-driven responses. They also show an asymmetry between the growth and differentiation induced by IL-2. This is reflected by opposite modulation on activation of PKC and by the role of the autocrine factor, IL-6.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1879879-2165880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1879879-2179132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1879879-2295801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1879879-2373516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1879879-2426188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1879879-2437013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1879879-2458941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1879879-2606140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1879879-2784412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1879879-2784752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1879879-2787350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1879879-2945860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1879879-2981272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1879879-3109220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1879879-3500860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1879879-3876180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1879879-6211485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1879879-6267587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1879879-6434631
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
73
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
322-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1879879-B-Lymphocytes,
pubmed-meshheading:1879879-Cells, Cultured,
pubmed-meshheading:1879879-DNA,
pubmed-meshheading:1879879-Enzyme Activation,
pubmed-meshheading:1879879-Forskolin,
pubmed-meshheading:1879879-Humans,
pubmed-meshheading:1879879-Immunoglobulin G,
pubmed-meshheading:1879879-Immunoglobulin M,
pubmed-meshheading:1879879-Interleukin-2,
pubmed-meshheading:1879879-Interleukin-6,
pubmed-meshheading:1879879-Ionomycin,
pubmed-meshheading:1879879-Lymphocyte Activation,
pubmed-meshheading:1879879-Palatine Tonsil,
pubmed-meshheading:1879879-Phorbol 12,13-Dibutyrate,
pubmed-meshheading:1879879-Protein Kinase C
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pubmed:year |
1991
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pubmed:articleTitle |
Interleukin-2-induced DNA synthesis and immunoglobulin secretion by resting human tonsillar B cells: effects of protein kinase C activation.
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pubmed:affiliation |
Immunodeficiency Diseases Research Group, Clinical Research Centre, Harrow, U.K.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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