| pubmed-article:1879657 | pubmed:abstractText | A series of experiments was carried out in immature female chicks and ducks to establish whether the avian Harderian gland contains specific receptors for sex steroids. Cytosol preparations of Harderian glands were submitted to hormone saturation analysis using radiolabeled estradiol, ORG-2058, and dimethylnortestosterone as ligands. In addition, the sedimentation characteristics of the hormone-receptor complexes were studied by ultracentrifugation of linear sucrose gradients. The presence of high affinity binding sites for estrogens (Kd = 2.4 and 1.6 nM), progestins (0.8 and 1.0 nM), and androgens (1.0 and 1.0 nM) was indicated in the chick and duck glands, respectively. The sedimentation coefficients were 7-7.5 S, 7-8 S, and 8 S for estrogen, progestin, and androgen receptor-ligand complexes, respectively. The concentration of the androgen receptor was significantly higher in chick than in duck Harderian glands whereas the estrogen and progestin receptor concentrations were similar in both species. A striking finding was the presence of progestin receptors, which apparently do not exist in the glands of many mammals. Priming with estrogens did not modify the concentration of ORG-2058 binding sites in either species studied, indicating that gland progestin receptor is not estrogen-regulated. Overall the data suggest intracellular mechanisms whereby circulating gonadal hormones regulate avian Harderian gland function. | lld:pubmed |
