Linked Life Data

18796227

Source:http://linkedlifedata.com/resource/pubmed/id/18796227

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2008-9-17
pubmed:abstractText
A high-performance liquid chromatographic method, coupled with UV detection and electrospray ionization mass spectrometry (HPLC-UV-ESI-MS), is developed for the simultaneous determination of the illegal additives sibutramine and its metabolite N-di-desmethylsibutramine in dietary supplements for weight control. The separation is achieved on a Spherisorb C8 reversed-phase column, employing acetonitrile and an aqueous 0.2% formic acid solution containing 20mM ammonium acetate as mobile phases in a gradient mode. UV detection is used for quantitation at a wavelength of 223 nm. Identification of target compounds is completed by ESI-MS using selected ion recording at m/z 280 for sibutramine and m/z 252 for N-di-desmethylsibutramine. Calibration curves are linear over the range of 0.025-1.0 mg/mL for sibutramine and N-di-desmethylsibutramine. Correlation coefficients are better than 0.9990. The intra- and inter-day precision and accuracy for sibutramine and N-di-desmethylsibutramine are acceptable. The method is successfully applied to the analysis of natural dietary supplement samples.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9665
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
707-11
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Simultaneous determination of sibutramine and N-Di-desmethylsibutramine in dietary supplements for weight control by HPLC-ESI-MS.
pubmed:affiliation
State Key Laboratory of Chemo/Biological Sensing & Chemometrics, Hunan University, Changsha 410082, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Validation Studies