Linked Life Data

1879495

Source:http://linkedlifedata.com/resource/pubmed/id/1879495

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1991-10-3
pubmed:abstractText
Fluorocitrate was injected in the vitreum of rats in order to define the experimental conditions for a temporary impairment of Müller cell metabolism in the retina. Injection of 16 nmol of fluorocitrate appeared to fulfil this requirement since this dose resulted in a large decrease in retinal endogenous glutamine and a smaller decrease in glutamate within 6 hr of administration. The reversible nature of the effect was attested by a substantial recovery of the retinal levels of the two amino acids within 24 hr of injection. In vitro experiments of carbon incorporation from different substrates, carried out with retinas dissected from eyes previously injected with fluorocitrate, were consistent with a metabolic impairment of glial cells, since carbon incorporation from [14C]acetate into glutamine was almost completely abolished in the fluorocitrate-treated retinas. Electron microscopic examination in fluorocitrate-poisoned retinas demonstrated essentially selective ultrastructural alterations of Müller cells at times corresponding to their maximal metabolic impairment. Since Müller cells are by far the largest glial population of the rat retina, common astrocytes being only scattered in the nerve fibre layer, the present experimental model may be used to study the role of Müller cells in the metabolism of retinal neurotransmitters.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0014-4835
pubmed:author
pubmed:issnType
Print
pubmed:volume
53
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
115-22
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Temporary impairment of Müller cell metabolism in the rat retina by intravitreal injection of fluorocitrate.
pubmed:affiliation
Department of Biology, University of Bologna, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't