Linked Life Data

18794862

Source:http://linkedlifedata.com/resource/pubmed/id/18794862

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pubmed-article:18794862pubmed:abstractTextWe introduce optogenetic investigation of neurotransmission (OptIoN) for time-resolved and quantitative assessment of synaptic function via behavioral and electrophysiological analyses. We photo-triggered release of acetylcholine or gamma-aminobutyric acid at Caenorhabditis elegans neuromuscular junctions using targeted expression of Chlamydomonas reinhardtii Channelrhodopsin-2. In intact Channelrhodopsin-2 transgenic worms, photostimulation instantly induced body elongation (for gamma-aminobutyric acid) or contraction (for acetylcholine), which we analyzed acutely, or during sustained activation with automated image analysis, to assess synaptic efficacy. In dissected worms, photostimulation evoked neurotransmitter-specific postsynaptic currents that could be triggered repeatedly and at various frequencies. Light-evoked behaviors and postsynaptic currents were significantly (P <or= 0.05) altered in mutants with pre- or postsynaptic defects, although the behavioral phenotypes did not unambiguously report on synaptic function in all cases tested. OptIoN facilitates the analysis of neurotransmission with high temporal precision, in a neurotransmitter-selective manner, possibly allowing future investigation of synaptic plasticity in C. elegans.lld:pubmed
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pubmed-article:18794862pubmed:year2008lld:pubmed
pubmed-article:18794862pubmed:articleTitleOptogenetic analysis of synaptic function.lld:pubmed
pubmed-article:18794862pubmed:affiliationInstitute of Biochemistry, Department of Biochemistry, Chemistry and Pharmacy, Goethe University Frankfurt, Biocenter N220, Frankfurt, Germany.lld:pubmed
pubmed-article:18794862pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:18794862pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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