Source:http://linkedlifedata.com/resource/pubmed/id/18794862
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
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pubmed:dateCreated |
2008-9-30
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pubmed:abstractText |
We introduce optogenetic investigation of neurotransmission (OptIoN) for time-resolved and quantitative assessment of synaptic function via behavioral and electrophysiological analyses. We photo-triggered release of acetylcholine or gamma-aminobutyric acid at Caenorhabditis elegans neuromuscular junctions using targeted expression of Chlamydomonas reinhardtii Channelrhodopsin-2. In intact Channelrhodopsin-2 transgenic worms, photostimulation instantly induced body elongation (for gamma-aminobutyric acid) or contraction (for acetylcholine), which we analyzed acutely, or during sustained activation with automated image analysis, to assess synaptic efficacy. In dissected worms, photostimulation evoked neurotransmitter-specific postsynaptic currents that could be triggered repeatedly and at various frequencies. Light-evoked behaviors and postsynaptic currents were significantly (P <or= 0.05) altered in mutants with pre- or postsynaptic defects, although the behavioral phenotypes did not unambiguously report on synaptic function in all cases tested. OptIoN facilitates the analysis of neurotransmission with high temporal precision, in a neurotransmitter-selective manner, possibly allowing future investigation of synaptic plasticity in C. elegans.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1548-7105
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
895-902
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pubmed:meshHeading |
pubmed-meshheading:18794862-Acetylcholine,
pubmed-meshheading:18794862-Animals,
pubmed-meshheading:18794862-Animals, Genetically Modified,
pubmed-meshheading:18794862-Caenorhabditis elegans,
pubmed-meshheading:18794862-Carrier Proteins,
pubmed-meshheading:18794862-Light,
pubmed-meshheading:18794862-Motor Neurons,
pubmed-meshheading:18794862-Muscle Contraction,
pubmed-meshheading:18794862-Muscle Relaxation,
pubmed-meshheading:18794862-Synapses,
pubmed-meshheading:18794862-Synaptic Transmission,
pubmed-meshheading:18794862-gamma-Aminobutyric Acid
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pubmed:year |
2008
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pubmed:articleTitle |
Optogenetic analysis of synaptic function.
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pubmed:affiliation |
Institute of Biochemistry, Department of Biochemistry, Chemistry and Pharmacy, Goethe University Frankfurt, Biocenter N220, Frankfurt, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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