Linked Life Data

18792075

Source:http://linkedlifedata.com/resource/pubmed/id/18792075

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2008-10-15
pubmed:abstractText
The homeostatic chemokine CXCL13 is preferentially produced in B-follicles and is crucial in the lymphoid organ development by attracting B-lymphocytes that express its selective receptor CXCR5. Follicular dendritic cells (FDCs) have been identified as the main cellular source of this chemokine in lymphoid organs. Recently, genome-wide approaches have suggested follicular CD4 T-helper cells (T(H)F) as additional CXCL13 producers in the germinal centre and the neoplastic counterpart of T(H)F (CD4+ tumour T-cells in angioimmunoblastic T-cell lymphoma) retains the capability of producing this chemokine. In contrast, no data are available on CXCL13 expression on FDC sarcoma (FDC-S) cells. By using multiple approaches, we investigated the expression of CXCL13 at mRNA and protein level in reactive and neoplastic FDCs. In reactive lymph nodes and tonsils, CXCL13 protein is mainly expressed by a subset of FDCs in B-cell follicles. CXCL13 is maintained during FDC transformation, since both dysplastic FDCs from 13 cases of Castleman's disease and neoplastic FDCs from ten cases of FDC-S strongly and diffusely express this chemokine. This observation was confirmed at mRNA level by using RT-PCR and in situ hybridization. Of note, no CXCL13 reactivity was observed in a cohort of epithelial and mesenchymal neoplasms potentially mimicking FDC-S. FDC-S are commonly associated with a dense intratumoural inflammatory infiltrate and immunohistochemistry showed that these lymphocytes express the CXCL13 receptor CXCR5 and are mainly of mantle zone B-cell derivation (IgD+ and TCL1+). In conclusion, this study demonstrates that CXCL13 is produced by dysplastic and neoplastic FDCs and can be instrumental in recruiting intratumoural CXCR5+ lymphocytes. In addition to the potential biological relevance of this expression, the use of reagents directed against CXCL13 can be useful to properly identify the origin of spindle cell and epithelioid neoplasms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1096-9896
pubmed:author
pubmed:copyrightInfo
(c) 2008 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
pubmed:issnType
Electronic
pubmed:volume
216
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
356-64
pubmed:dateRevised
2009-2-10
pubmed:meshHeading
pubmed-meshheading:18792075-Adolescent, pubmed-meshheading:18792075-Adult, pubmed-meshheading:18792075-Aged, pubmed-meshheading:18792075-Chemokine CXCL13, pubmed-meshheading:18792075-Dendritic Cells, Follicular, pubmed-meshheading:18792075-Female, pubmed-meshheading:18792075-Giant Lymph Node Hyperplasia, pubmed-meshheading:18792075-Humans, pubmed-meshheading:18792075-Immunohistochemistry, pubmed-meshheading:18792075-In Situ Hybridization, pubmed-meshheading:18792075-Lymph Nodes, pubmed-meshheading:18792075-Male, pubmed-meshheading:18792075-Middle Aged, pubmed-meshheading:18792075-Palatine Tonsil, pubmed-meshheading:18792075-RNA, Messenger, pubmed-meshheading:18792075-Receptors, CXCR5, pubmed-meshheading:18792075-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:18792075-Sarcoma, pubmed-meshheading:18792075-T-Lymphocytes, pubmed-meshheading:18792075-Tumor Markers, Biological
pubmed:year
2008
pubmed:articleTitle
Identification of CXCL13 as a new marker for follicular dendritic cell sarcoma.
pubmed:affiliation
Department of Pathology, Spedali Civili di Brescia, University of Brescia, Brescia, Italy. william.vermi@gmail.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't