Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
2008-10-20
pubmed:abstractText
Pseudomonas aeruginosa uses type IV pili to colonize various materials and for surface-associated twitching motility. We previously identified five phylogenetically distinct alleles of pilA in P. aeruginosa, four of which occur in genetic cassettes with specific accessory genes (J. V. Kus, E. Tullis, D. G. Cvitkovitch, and L. L. Burrows, Microbiology 150:1315-1326, 2004). Each of the five pilin alleles, with and without its associated pilin accessory gene, was used to complement a group II PAO1 pilA mutant. Expression of group I or IV pilA genes restored twitching motility to the same extent as the PAO1 group II pilin. In contrast, poor twitching resulted from complementation with group III or group V pilA genes but increased significantly when the cognate tfpY or tfpZ accessory genes were cointroduced. The enhanced motility was linked to an increase in recoverable surface pili and not to alterations in total pilin pools. Expression of the group III or V pilins in a PAO1 pilA-pilT double mutant yielded large amounts of surface pili, regardless of the presence of the accessory genes. Therefore, poor piliation in the absence of the TfpY and TfpZ accessory proteins results from a net increase in PilT-mediated retraction. Similar phenotypes were observed for tfpY single and tfpY-pilT double knockout mutants of group III strain PA14. A PilAV-TfpY chimera produced few surface pili, showing that the accessory proteins are specific for their cognate pilin. The genetic linkage between specific pilin and accessory genes may be evolutionarily conserved because the accessory proteins increase pilus expression on the cell surface, thereby enhancing function.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-10048019, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-10377148, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-10537208, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-10639466, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-10843854, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-10846211, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-10993081, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-11114900, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-11294863, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-11381130, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-11443078, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-12010970, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-12142488, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-12406226, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-12446837, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-12450816, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-12769840, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-1358873, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-14617778, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-15133094, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-15350129, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-15853879, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-16091031, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-16172128, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-16299283, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-16430686, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-16968224, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-17085575, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-17468398, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-17573479, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-17893339, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-18310333, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-18399938, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-1971258, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-1971619, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-2357375, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-2499765, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-2866848, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-7477282, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-7565109, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-7565110, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-7604262, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-7773418, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-7854122, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-7926843, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-7934852, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-8267974, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-8550441, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-8682785, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-8899718, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-8917091, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-8973346, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-9224879, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-9661666, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-9844000, http://linkedlifedata.com/resource/pubmed/commentcorrection/18776014-9882683
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1098-5530
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
190
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7022-34
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Novel proteins that modulate type IV pilus retraction dynamics in Pseudomonas aeruginosa.
pubmed:affiliation
Department of Biochemistry and Biomedical Sciences, The Michael G. DeGroote Institute for Infectious Diseases Research, Rm. 4H18, Health Sciences Centre, McMaster University, 1200 Main St. W., Hamilton, ON, Canada L8N 3Z5.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't