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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
17
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pubmed:dateCreated |
2008-9-5
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pubmed:abstractText |
Microtubule nucleation and formation from the kinetochore/chromatin have been proposed to contribute to bipolar spindle assembly facilitating equal segregation of chromosomes in mitosis. Although two independent pathways involving the small Ran GTPase-TPX2 proteins and the chromosomal passenger complex proteins have been implicated in the formation of microtubules from the kinetochore/chromatin, detailed molecular mechanisms integrating the pathways and regulating the process have not been well elucidated. This study demonstrates that Aurora kinase-A plays a central role in the kinetochore/chromatin associated microtubule assembly in human cells by integrating the two pathways regulating the process. Silencing by siRNA and over expression of a kinase inactive mutant revealed involvement of Aurora-A at two critical steps. These include accumulation of g-tubulin in the vicinity of kinetochore/chromatin to create microtubule nucleation sites as well as INCENP and TPX2 mediated activation of Aurora-A facilitating formation and stabilization of microtubules. The findings provide the first evidence of Aurora-A, in association with INCENP and TPX2, being a key regulator of kinetochore/chromatin associated microtubule formation in human cells.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/BIRC5 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Chromatin,
http://linkedlifedata.com/resource/pubmed/chemical/Chromosomal Proteins, Non-Histone,
http://linkedlifedata.com/resource/pubmed/chemical/INCENP protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Inhibitor of Apoptosis Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/KIF2C protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Kinesin,
http://linkedlifedata.com/resource/pubmed/chemical/Microtubule-Associated Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Tubulin,
http://linkedlifedata.com/resource/pubmed/chemical/aurora kinase
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1551-4005
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2691-704
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:18773538-Chromatin,
pubmed-meshheading:18773538-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:18773538-Enzyme Activation,
pubmed-meshheading:18773538-Feedback, Physiological,
pubmed-meshheading:18773538-HeLa Cells,
pubmed-meshheading:18773538-Humans,
pubmed-meshheading:18773538-Inhibitor of Apoptosis Proteins,
pubmed-meshheading:18773538-Kinesin,
pubmed-meshheading:18773538-Kinetochores,
pubmed-meshheading:18773538-Microtubule-Associated Proteins,
pubmed-meshheading:18773538-Microtubules,
pubmed-meshheading:18773538-Mitotic Spindle Apparatus,
pubmed-meshheading:18773538-Models, Biological,
pubmed-meshheading:18773538-Neoplasm Proteins,
pubmed-meshheading:18773538-Phosphorylation,
pubmed-meshheading:18773538-Protein Binding,
pubmed-meshheading:18773538-Protein-Serine-Threonine Kinases,
pubmed-meshheading:18773538-Tubulin
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pubmed:year |
2008
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pubmed:articleTitle |
Aurora kinase-A regulates kinetochore/chromatin associated microtubule assembly in human cells.
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pubmed:affiliation |
Department of Molecular Pathology, Division of Pathology and Laboratory Medicine, The University of Texas MD Anderson Cancer Center, Houston, Texas 77504, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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