Linked Life Data

18770803

Source:http://linkedlifedata.com/resource/pubmed/id/18770803

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-9-4
pubmed:abstractText
Telomere sequences at the end of chromosomes control somatic cell division; therefore, telomere length in a given cell population provides information about its replication potential. This unit describes a method for flow cytometric measurement of telomere length in subpopulations using fluorescence in situ hybridization of fluorescently-labeled probes (Flow-FISH) without prior cell separation. After cells are stained for surface immunofluorescence, antigen-antibody complexes are covalently cross-linked onto cell membranes before FISH with a telomere-specific probe. Cells with long telomeres are included as internal standards. Addition of a DNA dye permits exclusion of proliferating cells during data analysis. DNA ploidy measurements of cells of interest and internal standard are performed on separate aliquots in parallel to Flow-FISH. Telomere fluorescence of G(0/1) cells of subpopulations and internal standards obtained from Flow-FISH are normalized for DNA ploidy and telomere length in subsets of interest is expressed as a fraction of the internal standard telomere length.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1934-9300
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 7.26
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Assessment of telomere length, phenotype, and DNA content.
pubmed:affiliation
David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural