Source:http://linkedlifedata.com/resource/pubmed/id/18770603
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rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
2008-9-4
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pubmed:abstractText |
This unit includes protocols for the genetic manipulation of Mycobacterium tuberculosis, including nucleic acid extraction (plasmid DNA, genomic DNA, and mRNA), and methods for electroporation (transformation), transduction (including allelic exchange), and transposon mutagenesis. Considerations for working with M. tuberculosis at Biosafety Level 3 containment are also discussed.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1934-8533
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pubmed:author | |
pubmed:copyrightInfo |
Copyright 2007 by John Wiley & Sons, Inc.
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pubmed:issnType |
Electronic
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pubmed:volume |
Chapter 10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
Unit 10A.2
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pubmed:meshHeading |
pubmed-meshheading:18770603-DNA, Bacterial,
pubmed-meshheading:18770603-DNA Transposable Elements,
pubmed-meshheading:18770603-Electroporation,
pubmed-meshheading:18770603-Genetic Techniques,
pubmed-meshheading:18770603-Humans,
pubmed-meshheading:18770603-Mutagenesis,
pubmed-meshheading:18770603-Mycobacterium tuberculosis,
pubmed-meshheading:18770603-Transduction, Genetic
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pubmed:year |
2007
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pubmed:articleTitle |
Genetic Manipulation of Mycobacterium tuberculosis.
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pubmed:affiliation |
Albert Einstein College of Medicine, Bronx, New York, USA.
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pubmed:publicationType |
Journal Article
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