Linked Life Data

18770579

Source:http://linkedlifedata.com/resource/pubmed/id/18770579

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-9-4
pubmed:abstractText
Newcastle disease virus (NDV) is a prototype paramyxovirus used to define basic steps in the life cycle of this family of viruses. NDV is also an ideal virus system for elucidating determinants of viral pathogenicity. Some strains of this virus are important agricultural pathogens that cause disease in poultry with a high mortality while other strains are avirulent and used for vaccines. Methods for preparation and titration of virus stocks are essential for all of these purposes. Procedures for growth and purification of NDV stocks in embryonated chicken eggs as well as in tissue culture cells are described. Use of embryonated chicken eggs to grow the virus is the superior method since infectious stocks of all strains of NDV result. Stocks of avirulent NDV prepared in tissue culture are noninfectious. Virus stocks are routinely titered using plaque assays or hemagglutination assays, both of which are described.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1934-8533
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 15F.2
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Newcastle disease virus: propagation, quantification, and storage.
pubmed:affiliation
University of Massachusetts Medical School, Worcester, Massachusetts, USA.
pubmed:publicationType
Journal Article