Source:http://linkedlifedata.com/resource/pubmed/id/18770578
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
2008-9-4
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| pubmed:abstractText |
Detailed protocols are described for the propagation of adenoviruses (Ads) and adenovirus (Ad) vectors and their purification by CsCl equilibrium density gradient centrifugation. A discussion of monolayer and spinner cell culture techniques suitable, respectively, for small- and large-scale growth of adenoviruses is provided. Protocols for cloning into and growth of Ad replication-deficient vectors using a convenient commercially available system are described. Lastly, time-tested plaque titration protocols for the accurate and convenient measurement of the infectivity of adenoviruses and adenovirus vectors are provided in detail.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
1934-8533
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
Chapter 14
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
Unit 14C.1
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| pubmed:meshHeading | |
| pubmed:year |
2006
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| pubmed:articleTitle |
Human adenoviruses: propagation, purification, quantification, and storage.
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| pubmed:affiliation |
Saint Louis University School of Medicine, St Louis, Missouri, USA.
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| pubmed:publicationType |
Journal Article
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