Linked Life Data

18770560

Source:http://linkedlifedata.com/resource/pubmed/id/18770560

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-9-4
pubmed:abstractText
This unit describes the analysis of the translation-regulating properties of viral RNAs in cell-based assays using a luciferase reporter system. Electroporation and polyethylene glycol-mediated introduction of luciferase reporter RNA into cowpea protoplasts are also presented. The Commentary section discusses employing the luciferase reporter system to study translational control by the 5'- and 3'-untranslated regions of a viral mRNA. Inoculum RNA quality and half-life after delivery to the protoplasts should be monitored closely for careful investigation of translational regulation elements.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1934-8533
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 16K.2
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
In vivo translation studies of plant viral RNAs using reporter genes.
pubmed:affiliation
Oregon State University, Corvallis, Oregon, USA.
pubmed:publicationType
Journal Article