Source:http://linkedlifedata.com/resource/pubmed/id/18770535
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rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
2008-9-4
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pubmed:abstractText |
Potato virus X-based vectors are a well established system for rapid in planta studies. The vectors can be used for expression of proteins in plants and to down-regulate genes through virus-induced gene silencing. The development of binary-based vectors for Agrobacterium delivery makes this system well suited to high-throughput studies. Protocols are given for establishing infections to achieve expression and VIGS through in vitro transcription and Agrobacterium delivery to glasshouse and in vitro-grown plant material.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1934-8533
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
Chapter 16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
Unit 16I.1
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pubmed:meshHeading |
pubmed-meshheading:18770535-Gene Expression,
pubmed-meshheading:18770535-Gene Silencing,
pubmed-meshheading:18770535-Gene Transfer Techniques,
pubmed-meshheading:18770535-Genetic Vectors,
pubmed-meshheading:18770535-Plant Leaves,
pubmed-meshheading:18770535-Plant Tubers,
pubmed-meshheading:18770535-Plants,
pubmed-meshheading:18770535-Potexvirus,
pubmed-meshheading:18770535-Rhizobium,
pubmed-meshheading:18770535-Transformation, Genetic
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pubmed:year |
2008
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pubmed:articleTitle |
Use of potato virus X (PVX)-based vectors for gene expression and virus-induced gene silencing (VIGS).
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pubmed:affiliation |
Plant Pathology Programme, Scottish Crop Research Institute, Invergowrie, Dundee, United Kingdom.
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pubmed:publicationType |
Journal Article
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