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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1977-2-26
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pubmed:abstractText |
Lactoperoxidase-catalyzed radioiodination was used to study reactions between surface antigens and antibodies on BHK-21 cells infected with HSV-1 and HSV-2. Isolation of iodinated surface antigens was achieved by indirect immune precipitation of Triton X-100 disrupted cells with antisera to HSV and IgG. Analysis of immune precipitates by polyacrylamide gel electrophoresis (PAGE) revealed at least 10 antigens, ranging in m.w. from 35 x 103 to 160 x 103 daltons. Antigens were detectable on cell surfaces as early as 2 hr post-infection. Electrophoretic patterns of surface antigens induced by HSV-1 were similar to those induced by HSV-2. In both cases the major portion of activity was associated with glycoprotein(s) in the range of 115 x 103 to 130 x 103 daltons. A reduced amount of radioactivity was obtained if cells were reacted with anti-HSV sera before disruption with Triton X-100, suggesting that less surface antigen was accessible to HSV antibody applied directly to intact cells.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
118
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
114-21
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:187696-Antibodies, Viral,
pubmed-meshheading:187696-Antigen-Antibody Reactions,
pubmed-meshheading:187696-Antigens, Viral,
pubmed-meshheading:187696-Cell Line,
pubmed-meshheading:187696-Cell Membrane,
pubmed-meshheading:187696-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:187696-Glycoproteins,
pubmed-meshheading:187696-Molecular Weight,
pubmed-meshheading:187696-Simplexvirus,
pubmed-meshheading:187696-Time Factors,
pubmed-meshheading:187696-Virus Replication
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pubmed:year |
1977
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pubmed:articleTitle |
Immune interactions with cells infected with herpes simplex virus: antibodies to radioiodinated surface antigens.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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