Linked Life Data

18768977

Source:http://linkedlifedata.com/resource/pubmed/id/18768977

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
2008-10-27
pubmed:abstractText
CD4 T cells are not essential for primary clearance of replicating murine gammaherpesvirus 68 (MHV-68) but are required for effective long-term control. The virus reactivates in the lungs of major histocompatibility complex class II-deficient (CII-/-) mice that lack functional CD4 T cells. CD40 ligand (CD40L) is upregulated on activated CD4 T cells, and it is thought that CD40-CD40L interactions are an important component of CD4 T-cell help. Our previous studies have shown that agonistic antibodies to CD40 can substitute for CD4 T-cell function in the long-term control of MHV-68. In the present study, we sought to identify the CD40-positive cell type mediating this effect. To address this question, we adoptively transferred MHV-68 peptide-pulsed CII(-/-) dendritic cells (DC) that had been treated with an agonistic antibody to CD40 into MHV-68-infected CII(-/-) recipients. Viral reactivation was significantly lower in mice injected with anti-CD40-treated DC than in those injected with control DC or in mice that did not receive any DC. However, in similar experiments with B cells, anti-CD40 treatment had no effect. We also investigated the requirement for CD40 expression on T cells by adoptive transfer of T cells from CD40(+/+) or CD40(-/-) mice into T-cell-deficient recipients that were subsequently infected with MHV-68. The results showed that CD40 expression on T cells is not necessary for preventing viral reactivation. Taken together, our data suggest that CD40 engagement on DC, but not on T or B cells, is essential for effective long-term control of MHV-68.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-10074181, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-10384102, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-10395322, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-10395681, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-10516078, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-10878386, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-11313006, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-11353832, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-11458005, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-11751400, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-11861872, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-12242444, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-1328491, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-1469366, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-15286726, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-15584965, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-16212523, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-1677419, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-1692070, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-16934307, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-2161903, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-2351958, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-2955069, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-2981781, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-6111210, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-7508177, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-7583090, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-7997879, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-8885865, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-9031101, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-9064346, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-9223479, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-9298937, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-9389740, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-9624003, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-9624004, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-9624005, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-9625754, http://linkedlifedata.com/resource/pubmed/commentcorrection/18768977-9861009
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1098-5514
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11016-22
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
CD40 engagement on dendritic cells, but not on B or T cells, is required for long-term control of murine gammaherpesvirus 68.
pubmed:affiliation
Viral Immunology, Torrey Pines Institute for Molecular Studies, 3550 General Atomics Court, San Diego, CA 92121, USA. ssarawar@tpims.org
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural