18765740

Source:http://linkedlifedata.com/resource/pubmed/id/18765740

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0002812, umls-concept:C0005528, umls-concept:C0010837, umls-concept:C0033684, umls-concept:C0040557, umls-concept:C0205314, umls-concept:C0439794, umls-concept:C0679622, umls-concept:C1710548
pubmed:issue	11
pubmed:dateCreated	2008-10-20
pubmed:databankReference	http://linkedlifedata.com/resource/pubmed/xref/GENBANK/FJ015061
pubmed:abstractText	Toxoplasma gondii is an obligate intracellular parasite that resides in the cytoplasm of its host in a unique membrane-bound vacuole known as the parasitophorous vacuole (PV). The membrane surrounding the parasite is remodeled by the dense granules, secretory organelles that release an array of proteins into the vacuole and to the PV membrane (PVM). Only a small portion of the protein constituents of the dense granules have been identified, and little is known regarding their roles in infection or how they are trafficked within the infected host cell. In this report, we identify a novel secreted dense granule protein, GRA14, and show that it is targeted to membranous structures within the vacuole known as the intravacuolar network and to the vacuolar membrane surrounding the parasite. We disrupted GRA14 and exploited the knockout strain to show that GRA14 can be transferred between vacuoles in a coinfection experiment with wild-type parasites. We also show that GRA14 has an unexpected topology in the PVM with its C terminus facing the host cytoplasm and its N terminus facing the vacuolar lumen. These findings have important implications both for the trafficking of GRA proteins to their ultimate destinations and for expectations of functional domains of GRA proteins at the host-parasite interface.
pubmed:grant	http://linkedlifedata.com/resource/pubmed/grant/1R01AI064616, http://linkedlifedata.com/resource/pubmed/grant/5 P-30 DC006276-03, http://linkedlifedata.com/resource/pubmed/grant/T32-AI07323
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pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/0246127
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Protozoan Proteins
pubmed:status	MEDLINE
pubmed:month	Nov
pubmed:issn	1098-5522
pubmed:author	pubmed-author:BeckJosh RJR, pubmed-author:BradleyPeter JPJ, pubmed-author:RomeMichael EME, pubmed-author:TuretzkyJay MJM, pubmed-author:WebsterPaulP
pubmed:issnType	Electronic
pubmed:volume	76
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	4865-75
pubmed:dateRevised	2009-11-18
pubmed:meshHeading	pubmed-meshheading:18765740-Animals, pubmed-meshheading:18765740-Base Sequence, pubmed-meshheading:18765740-Blotting, Western, pubmed-meshheading:18765740-Cytoplasmic Granules, pubmed-meshheading:18765740-Fibroblasts, pubmed-meshheading:18765740-Host-Parasite Interactions, pubmed-meshheading:18765740-Humans, pubmed-meshheading:18765740-Microscopy, Fluorescence, pubmed-meshheading:18765740-Microscopy, Immunoelectron, pubmed-meshheading:18765740-Molecular Sequence Data, pubmed-meshheading:18765740-Polymerase Chain Reaction, pubmed-meshheading:18765740-Protein Transport, pubmed-meshheading:18765740-Protozoan Proteins, pubmed-meshheading:18765740-Toxoplasma, pubmed-meshheading:18765740-Toxoplasmosis, pubmed-meshheading:18765740-Vacuoles
pubmed:year	2008
pubmed:articleTitle	Intervacuolar transport and unique topology of GRA14, a novel dense granule protein in Toxoplasma gondii.
pubmed:affiliation	Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles CA 90095-1489, USA.

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