Linked Life Data

18761153

Source:http://linkedlifedata.com/resource/pubmed/id/18761153

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2008-9-1
pubmed:abstractText
This paper reported the coupling technique of Ag island film-enhanced fluorescence with rare earth co-luminescence effect of Tb-Gd-sodium dodecyl benzene sulfonate (SDBS)-protein system. While the collagen is used as the separator between Ag island film and the fluorophore because it not only can decrease the fluorescence of the blank, but also can promote the adsorption of other proteins and change the conformation of the protein. The effects of Ag island film on both the fluorescence and resonance energy transfer process of Tb-Gd-SDBS-protein system are studied, finding that Ag island film can enhance the energy transfer efficiency of this system, resulting in fluorescence enhancement about tenfold compared with this system without Ag island film. Therefore, this technique is used for the detection of proteins as low as 0.72 ng/mL for BSA and 1.3 ng/mL for HSA. In addition, Ag island film can also change the energy transfer process of Tb-SDBS-protein system.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1873-3573
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1047-52
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Ag island film-enhanced rare earth co-luminescence effect of Tb-Gd-protein-sodium dodecyl benzene sulfonate system and sensitive detection of protein.
pubmed:affiliation
Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't