Linked Life Data

18760499

Source:http://linkedlifedata.com/resource/pubmed/id/18760499

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-2-9
pubmed:abstractText
alpha-Dioxygenases are expressed in plants in response to biotic and abiotic stress. They catalyze the enantioselective 2-hydroperoxidation of long-chain fatty acids, the initial step of the alpha-oxidation pathway of fatty acids in plants. In this study, the complete cDNA of an alpha-dioxygenase from germinating pea seeds (Pisum sativum) is presented. The deduced amino acid sequence establishes that the enzyme belongs to the recently characterized family of alpha-dioxygenating enzymes in plants. We also present the first systematic study on the expression of alpha-dioxygenase in germinating and developing pea plants. During germination, alpha-dioxygenase mRNA accumulates in the cotyledons and the embryonic axis of pea seeds de novo. In developing pea plants, the transcript is detected almost exclusively in roots. The accumulation of alpha-dioxygenase protein parallels transcript accumulation in that it is abundant in germinating as well as young plant tissue, and correlates with loss of mRNA during plant maturation. alpha-Dioxygenase enzymatic activity in plant extracts is highest in cotyledons during imbibition. In the embryonic axis and roots of developing plants comparable activity levels are observed, whereas in shoots little alpha-oxidation activity is detected. With this contribution, we present information on the temporal and spatial expression of alpha-dioxygenase during plant germination and development, supporting the hypothesis that the alpha-oxidation pathway of fatty acids plays a role during plant developmental processes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1618-1328
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
166
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
333-43
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Fatty acid alpha-dioxygenase from Pisum sativum: temporal and spatial regulation during germination and plant development.
pubmed:affiliation
Institute of Pharmacy and Food Chemistry, University of Würzburg, Würzburg, Germany.
pubmed:publicationType
Journal Article