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rdf:type |
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lifeskim:mentions |
umls-concept:C0003241,
umls-concept:C0003501,
umls-concept:C0018826,
umls-concept:C0439611,
umls-concept:C0887869,
umls-concept:C1135183,
umls-concept:C1257975,
umls-concept:C1420728,
umls-concept:C1522408,
umls-concept:C1533691,
umls-concept:C1627358,
umls-concept:C1697272,
umls-concept:C1704806,
umls-concept:C2349975,
umls-concept:C2603343
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pubmed:issue |
1
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pubmed:dateCreated |
2009-1-5
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pubmed:abstractText |
The importance of cell adhesion to the scaffold in the tissue-engineered heart valve remains to be determined. The current study examined the feasibility of conjugating antibody against CD90 to a decellularized porcine aortic valve scaffold and binding mesenchymal stem cells to that scaffold through interaction with a cell surface antigen. After decellularization, the porcine aortic valve was reacted with biotin, avidin, and biotinylated anti-rat CD90 antibody sequentially and inserted into a laminar flow system used to test the effect of laminar shear stress. Rat bone mesenchymal stem cells (BMSC) were injected and circulated in a flow system to study the ability of anti-CD90 antibody to trap and immobilize cells on the valve surface. The results demonstrated that anti-CD90 antibody on the valve surface remains bound, even under high shear conditions. Compared with the control valve (no antibody), the modified (antibody-coated) valve immobilized significantly more rat BMSC (p < 0.05). Thus, the avidin-biotin system can be used to attach anti-CD90 antibody to these valves, and the bound antibody can immobilize rat BMSC in a flow chamber, suggesting that antibody-modified scaffolds might be used to fabricate shear stress-resistant, tissue-engineered heart valves.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1937-3341
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1-11
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pubmed:meshHeading |
pubmed-meshheading:18759669-Animals,
pubmed-meshheading:18759669-Antibodies, Anti-Idiotypic,
pubmed-meshheading:18759669-Aortic Valve,
pubmed-meshheading:18759669-Avidin,
pubmed-meshheading:18759669-Bioprosthesis,
pubmed-meshheading:18759669-Biotin,
pubmed-meshheading:18759669-Cell Adhesion,
pubmed-meshheading:18759669-Cells, Cultured,
pubmed-meshheading:18759669-Coated Materials, Biocompatible,
pubmed-meshheading:18759669-Equipment Design,
pubmed-meshheading:18759669-Feasibility Studies,
pubmed-meshheading:18759669-Formazans,
pubmed-meshheading:18759669-Heart Valve Prosthesis,
pubmed-meshheading:18759669-Mesenchymal Stem Cells,
pubmed-meshheading:18759669-Prosthesis Design,
pubmed-meshheading:18759669-Rats,
pubmed-meshheading:18759669-Shear Strength,
pubmed-meshheading:18759669-Sus scrofa,
pubmed-meshheading:18759669-Tetrazolium Salts,
pubmed-meshheading:18759669-Tissue Engineering,
pubmed-meshheading:18759669-Tissue Scaffolds
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pubmed:year |
2009
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pubmed:articleTitle |
Enhancement of mesenchymal stem cell attachment to decellularized porcine aortic valve scaffold by in vitro coating with antibody against CD90: a preliminary study on antibody-modified tissue-engineered heart valve.
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pubmed:affiliation |
Department of Cardiac Surgery, Institute of Cardiovascular Diseases, Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, P.R. China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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