Linked Life Data

18751697

Source:http://linkedlifedata.com/resource/pubmed/id/18751697

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2008-12-3
pubmed:abstractText
Ribosome inactivating proteins (RIPs) are enzymes that inactivate ribosomes by eliminating one or more adenosine residues from rRNA, a 9,567-Da RIP with a novel N-terminal sequence was isolated from fresh fruiting bodies of the mushroom Hypsizigus marmoreus. The protein was unadsorbed on DEAE-cellulose, adsorbed on Affi-gel blue gel, and appeared as a single peak upon gel filtration on Superdex 75. The protein, designated as marmorin, inhibited proliferation of hepatoma Hep G2 cells and breast cancer MCF-7 cells, HIV-1 reverse transcriptase activity, and translation in the rabbit reticulocyte lysate system with an IC50 of 0.15 microM, 5 microM, 30 microM, and 0.7 nM, respectively. Compared to RIPs from hairy gourd, bitter gourd, ridge gourd, garden pea, and the mushroom Flammulina velutipes, marmorin was more potent in its antiproliferative activity toward hepatoma (HepG2) and breast cancer (MCF-7) cells, similar in inhibitory potency toward HIV-1 reverse transcriptase (with the exception that it was more potent than ridge gourd RIP and bitter gourd RIP), and less potent in translation-inhibitory potency. Marmorin was devoid of antifungal, protease, RNase, mitogenic, anti-mitogenic, nitric oxide-inducing, hemagglutinating, and trypsin inhibitory activities.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1432-0614
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
81
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
669-74
pubmed:meshHeading
pubmed-meshheading:18751697-Agaricales, pubmed-meshheading:18751697-Amino Acid Sequence, pubmed-meshheading:18751697-Animals, pubmed-meshheading:18751697-Anti-HIV Agents, pubmed-meshheading:18751697-Cell Line, Tumor, pubmed-meshheading:18751697-Fruiting Bodies, Fungal, pubmed-meshheading:18751697-Fungal Proteins, pubmed-meshheading:18751697-Growth Inhibitors, pubmed-meshheading:18751697-HIV Reverse Transcriptase, pubmed-meshheading:18751697-Humans, pubmed-meshheading:18751697-Mice, pubmed-meshheading:18751697-Molecular Sequence Data, pubmed-meshheading:18751697-Molecular Weight, pubmed-meshheading:18751697-Momordica charantia, pubmed-meshheading:18751697-Reverse Transcriptase Inhibitors, pubmed-meshheading:18751697-Ribosome Inactivating Proteins, pubmed-meshheading:18751697-Sequence Alignment
pubmed:year
2008
pubmed:articleTitle
Marmorin, a new ribosome inactivating protein with antiproliferative and HIV-1 reverse transcriptase inhibitory activities from the mushroom Hypsizigus marmoreus.
pubmed:affiliation
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't