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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1991-9-24
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pubmed:abstractText |
A high-performance liquid chromatographic method with spectrophotometric detection was developed for the determination of proteins in urine. The proteins were separated on an anion-exchange column and eluted with a Tris-HCl buffer with a gradient of sodium chloride concentration and pH. The separated proteins were mixed with a pyrogallol red-molybdate complex reagent and determined spectrophotometrically. Urinary proteins were well separated without desalting the urine. The reproducibility was satisfactory.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9673
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
19
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pubmed:volume |
565
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
173-81
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:1874865-Chromatography, High Pressure Liquid,
pubmed-meshheading:1874865-Chromatography, Ion Exchange,
pubmed-meshheading:1874865-Humans,
pubmed-meshheading:1874865-Hydrogen-Ion Concentration,
pubmed-meshheading:1874865-Molybdenum,
pubmed-meshheading:1874865-Proteinuria,
pubmed-meshheading:1874865-Pyrogallol,
pubmed-meshheading:1874865-Retinol-Binding Proteins,
pubmed-meshheading:1874865-Sodium Chloride,
pubmed-meshheading:1874865-Spectrophotometry,
pubmed-meshheading:1874865-beta 2-Microglobulin
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pubmed:year |
1991
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pubmed:articleTitle |
Determination of proteins in urine by high-performance liquid chromatography with spectrophotometric detection using a pyrogallol red-molybdate complex.
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pubmed:affiliation |
Department of Laboratory Medicine, Faculty of Medicine, University of Tokyo, Japan.
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pubmed:publicationType |
Journal Article
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