rdf:type |
|
lifeskim:mentions |
umls-concept:C0010762,
umls-concept:C0012854,
umls-concept:C0017337,
umls-concept:C0017428,
umls-concept:C0038311,
umls-concept:C0319513,
umls-concept:C0332255,
umls-concept:C0475264,
umls-concept:C0599930,
umls-concept:C0679058,
umls-concept:C1547699,
umls-concept:C2700640
|
pubmed:issue |
3
|
pubmed:dateCreated |
1991-9-18
|
pubmed:databankReference |
|
pubmed:abstractText |
The gene encoding steroid inducible cytochrome P450 of Rhizopus nigricans ATCC 6227b has been found inside a HindIII fragment of the genomic DNA by hybridization with a partial length cDNA probe. The latter was isolated by immunoscreening a cDNA library prepared in the lambda gt11 expression system and identified on the basis of inducibility and sequence analysis. The nucleotide sequence of the cDNA probe revealed a coding sequence for the heme binding segment characteristic of the P450 gene family.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Aug
|
pubmed:issn |
0006-291X
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
15
|
pubmed:volume |
178
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1078-83
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:1872831-Amino Acid Sequence,
pubmed-meshheading:1872831-Base Sequence,
pubmed-meshheading:1872831-Cytochrome P-450 Enzyme System,
pubmed-meshheading:1872831-DNA, Fungal,
pubmed-meshheading:1872831-Deoxyribonuclease HindIII,
pubmed-meshheading:1872831-Gene Library,
pubmed-meshheading:1872831-Genes, Fungal,
pubmed-meshheading:1872831-Molecular Sequence Data,
pubmed-meshheading:1872831-Restriction Mapping,
pubmed-meshheading:1872831-Rhizopus,
pubmed-meshheading:1872831-Steroid Hydroxylases
|
pubmed:year |
1991
|
pubmed:articleTitle |
Localization of the gene encoding steroid hydroxylase cytochrome P-450 from Rhizopus nigricans inside a HindIII fragment of genomic DNA.
|
pubmed:affiliation |
Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Yugoslavia.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|